Skip to main content
Article thumbnail
Location of Repository

Characterization of cellular chemical dynamics using combined microfluidic and Raman techniques

By Xunli Zhang, Huabing Yin, Jon M. Cooper and Stephen J. Haswell

Abstract

The integration of a range of technologies including microfluidics, surface-enhanced Raman scattering and confocal microspectroscopy has been successfully used to characterize in situ single living CHO (Chinese hamster ovary) cells with a high degree of spatial (in three dimensions) and temporal (1 s per spectrum) resolution. Following the introduction of a continuous flow of ionomycin, the real time spectral response from the cell was monitored during the agonist-evoked Ca2+ flux process. The methodology described has the potential to be used for the study of the cellular dynamics of a range of signalling processes

Topics: QD, QH301
Year: 2008
OAI identifier: oai:eprints.soton.ac.uk:50092
Provided by: e-Prints Soton
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • http://dx.doi.org/10.1007/s002... (external link)
  • Suggested articles


    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.