<strong>Problem statement:</strong> An option to reduce pollution by synthetic agro-chemical in root plant disease management is the use of antagonist rhizobacteria belonging to <em>Bacillus</em> genus, because their inhibitory properties, stimulation of plant growth and crop yield increase. <strong>Approach:</strong> This study was carried out in order to evaluate if <em>Bacillus subtilis</em> strains could play an antagonists role of plant pathogens and if they can be microencapsulated inside a biopolymer matrix. It was adapted an equipment and evaluated a technique for microcapsules elaboration, in order to incorporate B. <em>subtilis</em> strains and to analyze their potential as biocontrol agents by determining their antagonistic effect against pathogenic soil fungi; in addition, it was analyzed their effect on tomato plant growth promotion under greenhouse conditions. B. <em>subtilis</em> strains identified as B1, J1, M2 and their mixture were used; microcapsules containing bacterial strains were inoculated to tomato seeds cv. Floradade. When seedlings emerged, a second application of microcapsules containing B. <em>subtilis</em> was performed on the pots, which previously were inoculated with the fungi<em> Rhizoctonia solani </em>and <em>Fusarium oxysporum</em>. Response variables were: Incidence and disease severity, plant growth, aerial and root dry weight, leaf area and fruit yield. <strong>Results:</strong> The outcome showed that the equipment designed and adapted for microcapsules elaboration was useful to obtain microcapsules containing the bacterial strains. B. <em>subtilis</em> strains exerted apparent biocontrol, since incidence and disease severity was reduced and for that reason inhibited the infective activity of the inoculated plant pathogens, also microcapsules containing Bacillus strains stimulated tomato growth and fruit yield. <strong>Conclusion:</strong> Microcapsules containing B. <em>subtilis</em> strains could be effective biocontrol agents against soil fungi plant pathogens and could have a potential biofertilizer effect, since they stimulated growth and yield of tomato plants compared to control
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