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Mitochondrial comparative proteomic analysis of sterile line and its maintain line of purple cytoplasmic rice (Oryza sativa)

By Peng Zhou, Zhenjiang Fei, Lei Wei, Jianhua Zhang, Hualin Dong and Xiaozhi Wu

Abstract

CMS/Rf systems in rice (Oryza sativa) have long been exploited for hybrid breeding to enhance productivity. Ying xiang CMS/Rf system is a new type. In this study, a mitochondrial comparative proteomic analysis of Ying xiang Sterile Line and its Maintain Line was started for a comprehensive investigation of the mitochondrial proteins’ functions in rice cytoplasmic male sterility. Mitochondria were prepared from rice shoots grown in the dark. Proteins were analyzed by two-dimensional electrophoresis and MALDI-TOF/ MS. Using Mascot, it was found that 7 proteins were not described previously for plant mitochondria, indicating novel mitochondrial functions. 3 of them were characterized

Topics: ATP Synthase, Comparative Proteomic Analysis, Cytoplasmic Male Sterile (CMS), Ying xiang A, Ying xiang B, LCC:Biology (General), LCC:QH301-705.5, LCC:Science, LCC:Q, DOAJ:Biology, DOAJ:Biology and Life Sciences
Publisher: Scientific Research Publishing
Year: 2010
DOI identifier: 10.4236/abb.2010.13020
OAI identifier: oai:doaj.org/article:050e44ae90814159965fcd282297f893
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