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The diagnostic importance of species specific and cross-reactive components of Taenia solium, Echinococcus granulosus, and Hymenolepis nana Importância diagnóstica da reação cruzada espécie-específica de componentes da Taenia solium, Echinococcus granulosus e Hymenolepis nana

By Teresa Montenegro, Robert H. Gilman, Rosa Castillo, Victor Tsang, Joy Brandt, Angela Guevara, Hernan Sanabria, Manuela Verastegui, Charles Sterling and Elba Miranda

Abstract

Sera from patients infected with Taenia solium, Hymenolepis nana and Echinococcus granulosus were tested against homologous and heterologous parasite antigens using an ELISA assay, and a high degree of cross-reactivity was verified. To identify polypeptides responsible for this cross reactivity, the Enzyme Linked Immunoelectro Transfer Blot (EITB) was used. Sera from infected patients with T.solium, H.nana, and E.granulosus were assessed against crude, ammonium sulphate precipitated (TSASP), and lentil-lectin purified antigens of T.solium and crude antigens of.H.nana and E.granulosus. Several bands, recognized by sera from patients with T.solium, H.nana, and E.granulosus infections, were common to either two or all three cestodes. Unique reactive bands in H.nana were noted at 49 and 66 K-Da and in E.granulosus at 17-21 K-Da and at 27-32 K-Da. In the crude cysticercosis extract, a specific non glycoprotein band was present at 61-67 K-Da in addiction to specific glycoprotein bands of 50, 42, 24, 21, 18, 14, and 13 K-Da. None of the sera from patients with H.nana or E.granulosus infection cross reacted with these seven glycoprotein bands considered specific for T.solium infection.<br>Soros de pacientes infectados com Taenia solium, Hymenolepis nana e Echinococcus granulosus foram testados contra antígenos parasitários homólogos e heterólogos usando o teste de ELISA e foi verificado alto grau de reatividade cruzada. Para identificar os polipetídeos responsáveis por esta reatividade cruzada foi utilizado o teste "Enzyme Linked Immunoelectro Transfer Blot (EITB)". Soros de pacientes infectados por T.solium, H.nana, e E.granulosus foram colocados em contato com precipitado de sulfato de amônia e antígenos não purificados de T.solium e os de H.nana e E.granulosus. Várias bandas reconhecidas pelos soros de pacientes com infecção por T.solium, H.nana e E.granulosus foram comuns a dois ou três destes cestódeos. Uma única banda foi notada em H.nana a 49 e 66K-Da e no E.granulosus a 17-21 K-Da e 27-32 K-Da. No extrato não purificado de cisticercose uma banda específica não glicoproteica estava presente a 61-67 K-Da além das bandas de glicoproteínas específicas de 50, 42, 24, 21, 18, 14 e 13 K-Da. Nenhum destes soros de pacientes com infecção por H.nana ou E.granulosus reagiu de forma cruzada com estas sete bandas de glicoproteína consideradas específicas à infecção por T.soliu

Topics: Taenia solium, Cysticercus, antigens, Hymenolepis nana, Echinococcus granulosus, Hydatid Cysts, SDS-PAGE, Electrophoresis, Immunoblotting, LCC:Medicine (General), LCC:R5-920, LCC:Medicine, LCC:R, DOAJ:Medicine (General), DOAJ:Health Sciences
Publisher: Instituto de Medicina Tropical
Year: 1994
DOI identifier: 10.1590/S0036-46651994000400005
OAI identifier: oai:doaj.org/article:1501acbc05334ff1bc316a9393bfcf6b
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