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Preliminary study towards a novel experimental model to study localized cutaneous leishmaniasis caused bY Leishmania (Leishmania) mexicana Estudio preliminar sobre un nuevo y original modelo experimental de leishmaniosis cutánea localizada causada por Leishmania (Leishmania) mexicana

By Erika Ivett Sosa-Bibiano, Nicole Raymonde Van Wynsberghe, Silvia Beatriz Canto-Lara and Fernando Jose Andrade-Narvaez

Abstract

There is not an experimental model of localized cutaneous leishmaniasis (LCL) caused by Leishmania (Leishmania) mexicana. The aim of the present study was to characterize the clinical and histological features of Peromyscus yucatanicus experimentally infected with L. (L.) mexicana. A total of 54 P. yucatanicus (groups of 18) were inoculated with 1x10(6) promastigotes of L. (L.) mexicana in the base of the tail. They were euthanized at three and six months post experimental infection. The control group was inoculated with RPMI-1640. The predominant clinical sign observed was a single ulcerated lesion in 27.77% (5/18) and in 11.11% (2/18) P. yucatanicus at three and six months respectively. The histological pattern described as chronic granulomatous inflammation with or without necrosis was found in 7/7 (100%) biopsies of euthanized P. yucatanicus at three (n = 5) and six (n = 2) months, respectively. These results resembled clinical and histological features caused by L. (L.) mexicana in humans, and support the possibility to employ P. yucatanicus as a novel experimental model to study LCL caused by this parasite.<br>No existe un modelo experimental de la leishmaniosis cutánea localizada (LCL) causada por Leishmania (Leishmania) mexicana. El objetivo del presente estudio fue el de caracterizar los cuadros clínico e histológico de Peromyscus yucatanicus infectados experimentalmente con L. (L.) mexicana. Un total de 54 P. yucatanicus (grupos de 18) fueron inoculados en la base de la cola con 1x10(6) promastigotes de L. (L.) mexicana. A los 3 y 6 meses posteriores a la infección experimental fueron sacrificados. El grupo control fue inoculado con RPMI-1640. El signo clínico predominante fue una lesión única ulcerada en 27.77% (5/18) y en 11.11% (2/18) P. yucatanicus a los 3 y 6 meses respectivamente. El patrón histológico descrito como inflamación crónica granulomatosa con o sin necrosis se observó en 7/7 (100%) biopsias de los P. yucatanicus a los 3 (n=5) y 6 (n=2) meses respectivamente. Los resultados son similares a los cuadros clínico e histológico de la infección por L. (L.) mexicana en humanos, y apoyan la posibilidad de utilizar P. yucatanicus como un nuevo y original modelo para el estudio de la LCL causada por L. (L.) mexicana

Topics: Peromyscus yucatanicus, Leishmania mexicana, Symptoms, Histology, LCC:Medicine (General), LCC:R5-920, LCC:Medicine, LCC:R, DOAJ:Medicine (General), DOAJ:Health Sciences
Publisher: Instituto de Medicina Tropical
Year: 2012
DOI identifier: 10.1590/S0036-46652012000300009
OAI identifier: oai:doaj.org/article:7d75a4059fbc40809f902e5280d60f6d
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