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Stock indexing and Potato virus Y elimination from potato plants cultivated in vitro Indexação de matrizes e eliminação do Potato virus Y em plantas de batata cultivadas in vitro

By Luciana Cordeiro Nascimento, Gilvan Pio-Ribeiro, Lilia Willadino and Genira Pereira Andrade

Abstract

Potato cultivars (Solanum tuberosum L.) have shown degeneration or run out caused by viruses after several cycles of propagation using seed tubers from commercial fields. This work reports the occurrence of single and mixed infections of four potato viruses in Paraíba-Brazil and presents a method for Potato virus Y (PVY) elimination, by using thermo-and chemotherapies. Plants of potato cv. Baraka were tested by direct antigen coating ELISA. Antisera against PVY, Potato virus X (PVX), Potato virus S (PVS), and Potato leafroll virus (PLRV) were used. Materials with positive reaction to PVY were treated for virus elimination. Single node cuttings (1.0 cm length) were excised and inoculated in Murashige & Skoog (MS) medium, supplemented with 1.0 mg L-1 of kinetin, 0.001 mg L-1 of naphthalene acetic acid (NAA) and 0.1 mg L-1 of gibberellic acid (GA3). The thermotherapy at approximately 37ºC, during 30 and 40 days, resulted in 20.0 and 37.5% PVY elimination, respectively. Chemotherapy was undertaken with Ribavirin (RBV), 5-Azacytidine (AZA), and 3-Deazauridine (DZD). The RBV showed the highest rate of virus eradication, with 55.5% virus-free plants. Simultaneous thermo and chemotherapy had higher efficiency for the elimination of PVY, reaching rates of healthy plants of 83.3% with RBV, 70.0% with AZA, and 50.0% with DZD.<br>Cultivares de batata (Solanum tuberosum L.) têm mostrado degenerescência causada por vírus após ciclos sucessivos do uso de tubérculos de campos comerciais como material propagativo. Este trabalho verifica a ocorrência de infecção simples e mista de quatro vírus da batata na Paraíba e apresenta adequação da técnica de cultivo in vitro para obtenção de material livre de Potato virus Y (PVY), incluindo uso de microestacas, termo e quimioterapia. Plantas de batata do cv. Baraka foram submetidas à indexação sorológica pelo teste "direct antigen coating" ELISA. Utilizaram-se antissoros contra o PVY, Potato virus X (PVX), Potato virus S (PVS) e Potato leafroll virus (PLRV). Materiais com reação positiva para PVY foram submetidos a tratamentos visando à eliminação viral. Microestacas (1,0 cm de comprimento) com uma gema foram excisadas e inoculadas em meio nutritivo de Murashige & Skoog (MS), suplementado com 1,0 mg L-1 de cinectina, 0,00l mg L-1 de ácido naftaleno acético (ANA) e 0,1 mg L-1 de ácido giberélico (GA3). Termoterapia a 37ºC, durante 30 e 40 dias, promoveu a eliminação do PVY em 20,0 e 37,5% no material testado, respectivamente. A quimioterapia foi realizada com Ribavirin (RBV), 5-Azacitidina (AZA) e 3-Deazauridine (DZD). O RBV apresentou os melhores índices de erradicação de vírus com a obtenção de 55,5% de plantas sadias. Tratamentos simultâneos de termo e quimioterapia mostraram maior eficiência na eliminação viral, atingindo um percentual de plantas sadias da ordem de 83,3 com RBV, 70,0 com AZA e 50,0 com DZD

Topics: Solanum tuberosum, limpeza clonal, termoterapia, quimioterapia, Solanum tuberosum, virus-free clones, thermotherapy, chemotherapy, LCC:Agriculture (General), LCC:S1-972, LCC:Agriculture, LCC:S, DOAJ:Agriculture (General), DOAJ:Agriculture and Food Sciences
Publisher: São Paulo - Escola Superior de Agricultura "Luiz de Queiroz"
Year: 2003
DOI identifier: 10.1590/S0103-90162003000300017
OAI identifier: oai:doaj.org/article:45d562a7c6294d1eb45bbef6b6850a5b
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