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Histoquímica de mucinas y lípidos del aparato urinario de la baba (Caiman crocodilus crocodilus) Mucins and Lipids Histochemistry of the Urinary System in the Spectacled Cayman (Caiman crocodilus crocodilus)

By Francisco J Cabrera A and Gisela C García C

Abstract

Se realizó un estudio histoquímico de mucinas y lípidos del aparato urinario de nueve babas adultas de sexo masculino y de un ejemplar adulto de sexo femenino, utilizando las coloraciones PAS, PAS-Azul alciano a pH 2,5 y 1,0; Aceite Rojo “O” y el Método de Schulz para esteroides. Los hallazgos más relevantes fueron la presencia de mucinas neutras en el borde en cepillo y  gotas citoplasmáticas de todos los túbulos de la nefrona y en la superficie apical del urotelio, así como presencia de sialomucinas desde el túbulo distal hasta el uréter. Se detectaron gotas lipídicas compuestas por ácidos grasos libres en el citoplasma de las células del segmento intermedio de la nefrona. Se discute la importancia de estas mucinas como protectores de las mucosas y como lubricantes de las vías urinarias. Estos estudios parecen demostrar la ausencia de porciones de la nefrona del Caiman crocodilus crocodilus que intervengan en la secreción de líquido seminal o que sean análogos del segmento sexual de los lagartos y serpientes.<br>A histochemical study of mucins and lipids of the urinary tract of the spectacled caiman was conducted, using PAS stain, PAS-Alcian blue stain (at pH values of 2.5 and 1.0, respectively), “O” red oil stain, and Shultz method for steroids. Nine mature males and one mature female were used. The results of the present study show that the most relevant findings were the presence of neutral mucins in the brush border and of cytoplasmic droplets in all tubular nephrons and in the apical surface of the urothelium, as well as the presence of sialomucins from the distal tubule to the ureter. Lipid droplets composed of free fatty acids were observed in the cytoplasm of the cells of the intermediate segment of the nephron. The importance of mucins as mucosal protectors and urinary tract lubricants is discussed. The results of the study in the Caiman crocodilus crocodilus, appear to demonstrate the absence of portions of the nephron that either participate in the secretion of seminal fluid or are similar to the sexual segment of lizards and snakes

Topics: Vías urinarias, mucinas, lípidos, histoquímica, Caiman crocodilus crocodilus, Apure, Urinary pathways, mucins, lipids, histochemistry, Caiman crocodilus crocodilus, Apure, LCC:Animal culture, LCC:SF1-1100, LCC:Agriculture, LCC:S, DOAJ:Animal Sciences, DOAJ:Agriculture and Food Sciences
Publisher: Revista de la Facultad de Ciencias Veterinarias. Universidad Central de Venezuela.
Year: 2010
OAI identifier: oai:doaj.org/article:5dcad99d68b7489186febade3cdd0d2e
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