Location of Repository

Nitrogen fixation in the western English Channel (NE Atlantic Ocean)

By Andrew P. Rees, Jack A. Gilbert and Boris A. Kelly-Gerreyn


In temperate Atlantic waters (18.8 to 20.1°C), biological nitrogen fixation has been<br/>demonstrated by 2 independent measurements: 15N-N2 incorporation and nifH identification in the<br/>DNA and expressed messenger RNA (mRNA). At 2 stations in the western English Channel, bulk<br/>waters were incubated with 15N-N2. At the high levels of particulate nitrogen (?11.5 ?mol N l–1),<br/>absolute fixation rates of 18.9 ± 0.01 and 20.0 nmol N l–1d–1 were determined. While a caveat must<br/>accompany the magnitude of the rates presented due to the limited number of data, the presence and<br/>activity of diazotrophic organisms in these waters is of ecological significance and may affect current<br/>attitudes to nitrogen and carbon budgets. In particular, our estimate of the rate of N fixation<br/>(0.35 mmol N m–2 d–1) is comparable to that of denitrification rates in UK shelf seas. Molecular analysis<br/>identified a diversity of expressed nifH genes, and 21 different prokaryotic nifH transcripts were<br/>identified

Topics: GC, GE, Q1
Year: 2009
OAI identifier: oai:eprints.soton.ac.uk:63462
Provided by: e-Prints Soton

Suggested articles



  1. (1982). 178:143–148 Davey A
  2. (2005). Comparison of nitrogen fixation rates determined with different methods: a study
  3. (2001). Distribution of nitrogenfixing microorganisms along the Neuse River Estuary, North Carolina. Microb Ecol 41:114–123
  4. (2002). Isolation of viruses responsible for the demise of an Emiliania huxleyi bloom in the English Channel.
  5. (2004). Patterns of nitrogen fixation along 10°N in the tropical Atlantic.
  6. (2007). Stable-isotope probing implicates Methylophaga spp. and novel Gammaproteobacteria in marine methanol and methylamine metabolism.
  7. (1999). The balance of supply of nutrients and demands of biological production and denitrification in a temperate latitude shelf sea—a treatment of the southern North Sea as an extended estuary. Mar Chem 68:117–131 Kelly-Gerreyn

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.