Article thumbnail

The use of whole genome and next-generation sequencing in the diagnosis of invasive fungal disease

By Sam (S34932) El-Kamand, Alexie (R18102) Papanicolaou and Charles O. (R16967) Morton

Abstract

Purpose of Review This review examines how next-generation nucleic acid sequencing (NGS) is being used in the diagnosis of invasive fungal disease (IFD) and how well its implementation compares to PCR-based diagnosis. The comparison allows for the assessment of the advantages and potential limitations of NGS in the diagnosis of IFD. Recent Findings NGS is established as a method for sequencing of microbial genomes. It is increasingly being tested as a direct diagnostic method from various sample types including samples such as plasma, formalin fixed and paraffin embedded specimens, and bronchoalveolar lavage. In these cases, the methodology was generally able to identify the causative agents of IFD. Summary NGS-based methods will play an increasingly important role in diagnosis of IFD. The major limitation is currently cost, the need to standardise methods of nucleic acid isolation, and sequence analysis to enable broad uptake and application of the method

Topics: 060505 - Mycology, 110899 - Medical Microbiology not elsewhere classified, 920109 - Infectious Diseases, mycoses, Aspergillosis, diagnosis
Publisher: U.S., Springer Healthcare
Year: 2019
DOI identifier: 10.1007/s12281-019-00363-5
OAI identifier: oai:nuws:uws_53747
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • https://hdl.handle.net/1959.7/... (external link)
  • https://ezproxy.uws.edu.au/log... (external link)
  • Suggested articles


    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.