Article thumbnail

A murine model of xenotransplantation of human glioblastoma with imunosupression by orogastric cyclosporin

By Alexandre M. Cunha, Fernanda S. Nascimento, Jane C.O.F. Amaral, Sandra Konig, Crhistina M. Takiya, Vivaldo M. Neto, Eduardo Rocha and Jorge P.B.M. Souza

Abstract

Several animal experimental models have been used in the study of malignant gliomas. The objective of the study was to test the efficacy of a simple, reproducible and low cost animal model, using human cells of glioblastoma multiforme (GBM) xenotransplantated in subcutaneous tissue of Wistar rats, immunosuppressed with cyclosporin given by orogastric administration, controlled by nonimunosuppressed rats. The animals were sacrificed at weekly intervals and we have observed gradual growth of tumor in the immunosuppressed group. The average tumor volume throughout the experiment was 4.38 cm³ in the immunosuppressed group, and 0.27 cm³ in the control one (p<0.001). Tumors showed histopathological hallmarks of GBM and retained its glial identity verified by GFAP and vimentin immunoreaction. Immunosuppression of rats with cyclosporin was efficient in allowing the development of human glioblastoma cells in subcutaneous tissues. The model has demonstrated the maintenance of most of the histopathological characteristics of human glioblastoma in an heterotopic site and might by considered in research of molecular and proliferative pathways of malignant gliomas

Topics: modelo animal, ratos Wistar, ciclosporina, glioblastoma, xenotransplante, Neurosciences. Biological psychiatry. Neuropsychiatry, RC321-571
Publisher: Academia Brasileira de Neurologia (ABNEURO)
Year: 2011
DOI identifier: 10.1590/S0004-282X2011000100021
OAI identifier: oai:doaj.org/article:343524f1efe54397bce0f030cbcb74b2
Journal:
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • https://doaj.org/toc/1678-4227 (external link)
  • http://www.scielo.br/scielo.ph... (external link)
  • https://doaj.org/article/34352... (external link)
  • Suggested articles


    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.