Purpose: This study aims at specifying the relationship between workplace spirituality and job performance among Isfahan Azad Islamic university's staff. Methodology: This study is descriptive correlational one conducted in 2015. Research population were all Isfahan Azad University staff, 375 individuals among the 189 ones were accidently and based on the mass of their department, and Cochran sample mass selected. Instruments used in this study were Ashmos (2000)'s 22-item questionnaire of workplace spirituality and Paterson's (1970) 15-item questionnaire of job performance. The stability of these questionnaires in Cronbach's Alpha was computed as 0.88 and 0.83 for workplace spirituality and job performance, respectively. Data extracted in this study was analyzed through multi-regression tests, Pierson correlation coefficient and SPSS software of variance analytical tests. Main Findings: The results of this study show that there exists a meaningful relation between workplace spirituality (r=0.330), meaningful work (r=0.287), a sense of connection and positive social relations with coworkers (r=0. 298), individual's alignment with the organization's values (r=0.326) and job performance. (P=0.001) in the first step, from among these workplace spirituality dimensions, meaningful work is the best indicator of staff's job performance. Implications: This study and results can throw light on strategies to be adopted to enhance job performance in University staff. Originality: This type of study is done first time in University.