A highly potent agonist to protease-activated receptor-2 reveals apical activation of the airway epithelium resulting in Ca²⁺-regulated ion conductance

Abstract

The airway epithelium provides a barrier that separates inhaled air and its various particulates from the underlying tissues. It provides key physiological functions in both sensing the environment and initiating appropriate innate immune defenses to protect the lung. Protease-activated receptor-2 (PAR2) is expressed both apically and basolaterally throughout the airway epithelium. One consequence of basolateral PAR2activation is the rapid, Ca2+-dependent ion flux that favors secretion in the normally absorptive airway epithelium. However, roles for apically expressed PAR2activation have not been demonstrated, in part due to the lack of specific, high-potency PAR2ligands. In the present study, we used the newly developed PAR2ligand 2at-LIGRLO( PEG3-Pam)-NH2in combination with well-differentiated, primary cultured airway epithelial cells from wild-type and PAR2−/−mice to examine the physiological role of PAR2in the conducting airway after apical activation. Using digital imaging microscopy of intracellular Ca2+concentration changes, we verified ligand potency on PAR2in primary cultured airway cells. Examination of airway epithelial tissue in an Ussing chamber showed that apical activation of PAR2by 2at-LIGRLO( PEG3-Pam)-NH2resulted in a transient decrease in transepithelial resistance that was due to increased apical ion efflux. We determined pharmacologically that this increase in ion conductance was through Ca2+-activated Cl−and large-conductance K+channels that were blocked with a Ca2+-activated Cl−channel inhibitor and clotrimazole, respectively. Stimulation of Cl−efflux via PAR2activation at the airway epithelial surface can increase airway surface liquid that would aid in clearing the airway of noxious inhaled agents.</jats:p