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Comparison of heat-shock responses between the hydrothermal vent shrimp Rimicaris exoculata and the related coastal shrimp Palaemonetes varians

By Delphine Cottin, Bruce Shillito, Thomas Chertemps, Sven Thatje, Nelly Leger and Juliette Ravaux

Abstract

The deep-sea vent shrimp Rimicaris exoculata is believed to occur at the hot end of the hydrothermal biotope in order to provide essential elements to its epibiosis. Because it is found close to hot venting water, R. exoculata lives in a highly fluctuating environment where temperature (2–40 °C in the swarms) can exceed its critical maximal temperature (33–38.5 ± 2 °C). In order to understand how this vent shrimp copes with hyperthermia, we compared its molecular heat stress response following an acute but non-lethal heat-shock (1 h at 30 °C) with that of its monophyletic shallow-water relative, the shrimp Palaemonetes varians, known to frequently undergo prolonged exposure at temperatures up to 30 °C in its natural environment during summer. We isolated four isoforms of heat-shock proteins 70 (HSP70) in R. exoculata (2 constitutive and 2 inducible isoforms) and two isoforms in P. varians (1 constitutive and 1 inducible isoform) and quantitatively compared their magnitude of induction at mRNA level, using real-time PCR, in the case of experimentally heat-stressed shrimps, with respect to control (unstressed) animals. Here, we report the first quantification of the expression of multiple hsp70 genes following heat stress in a deep-sea vent species living at 2300 m depth. Our results show a strong increase of hsp70 inducible genes in the vent shrimp (not, vert, similar 400-fold) compared to the coastal shrimp (not, vert, similar 15-fold). We therefore propose that, the highly inducible molecular response observed in R. exoculata may contribute to the ability of this species to tolerate thermal extremes

Topics: QH301, GC
Year: 2010
OAI identifier: oai:eprints.soton.ac.uk:72328
Provided by: e-Prints Soton

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Citations

  1. (2010). 9–16Author's personal copy
  2. (2000). A review of the distribution of hydrothermal vent communities along the Northern Mid-Atlantic Ridge: dispersal vs environmental controls. doi
  3. (2003). A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. doi
  4. (2000). Absolute quanti cation of mRNA using real-time reverse transcription polymerase chain reaction assays. doi
  5. (2008). Antartic marine molluscs do have an HSP70 heat shock response. doi
  6. (1992). Biochemical ecology of deep-sea animals. doi
  7. (1993). Chemosynthetic microbial activity at Mid-Atlantic Ridge hydrothermal vent sites. doi
  8. (2005). Comparative expression analysis of two paralogous Hsp70s in rainbown trout cells exposed to heat stress. doi
  9. (2004). Distribution of bacteria and associated minerals in the gill chamber of the vent shrimp Rimicaris exoculata and related biogeochemical processes. doi
  10. (1989). Effects of salinity changes on zinc uptake and regulation by the decapod crustaceans Palaemonetes elegans and Palaemonetes varians. doi
  11. (1998). Effects of short-term hypoxia on metabolism and haemocyanin oxygen transport in the prawns Palaemonetes adspersus and Palaemonetes varians. doi
  12. (2007). Expression of heat shock protein 70 in the thermally stressed Antartic clam Laternula elliptica. doi
  13. (1988). Feeding biology of the shrimp Rimicaris exoculata at hydrothermal vents on the Mid-Atlantic Ridge. doi
  14. (1993). Feeding specialization of bresiliid shrimps in the TAG site hydrothermal community. doi
  15. (2007). Field and laboratory investigations of the thermal in uence on tissue-speci c Hsp70 levels in common carp (Cyprinus carpio). doi
  16. (2007). Firsthsp70 from two hydrothermal vent shrimps, Mirocaris fortunata and Rimicaris exoculta: characterization and sequence analysis. doi
  17. (2000). Food source, behaviour and distribution of hydrothermal vent shrimps at the Mid-Atlantic Ridge. doi
  18. (2004). Genomic approaches to detecting thermal stress in Calanus nmarchicus (Copepoda: doi
  19. (2009). Global depression in gene expression as a response to rapid thermal changes in vent mussels. doi
  20. (1982). Heat shock and recovery are mediated by different translational mechanisms. doi
  21. (2009). Heat shock protein (Hsp) gene responses of the intertidal copepod Tigriopus japonicus to environmental toxicants. doi
  22. (2007). Heat shock protein expression pattern (HSP70) in the hydrothermal vent mussel Bathymodiolus azoricus. doi
  23. (2003). Heat shock response and temperature resistance in the deep-sea vent shrimp Rimicaris exoculata. doi
  24. (1999). Heat-shock proteins, molecular chaperones, and the stress response: evolutionary and ecological physiology. doi
  25. (2005). Hsp70 chaperones: cellular functions and molecular mechanism. doi
  26. (2006). Hsp70 gene expression in Mytilus galloprovincialis hemocytes is triggered by moderate heat-shock and Vibrio anguillarum, but not by V. splendidus or Micrococcus lysodeikticus. doi
  27. (2009). Hydrothermal shrimps display low expression of heat-inducible hsp70 gene in nature. doi
  28. (1991). Hydrothermal vent biology: ecology and evolution. doi
  29. (2007). Identi cation of cDNAs encoding HSP70 and HSP90 in the abalone Haliotis tuberculata: transcriptional induction in response to thermal stress in hemocyte primary culture. doi
  30. (2008). Interactions of deep-sea vent invertebrates with their environment: the case of Rimicaris exoculata. doi
  31. (1993). L'énigme du comportement trophique des crevettes Alvinocarididea des sites hydrothermaux de la dorsale médio-atlantique.
  32. (1997). Long-term changes in a brackish lagoon, Lady's Island lake, South-east Ireland. Biology and Environment.
  33. (2001). Microhabitats, thermal heterogeneity, and patterns of physiological stress in the rocky intertidal zone. doi
  34. (2004). Molecular cloning and expression of two doi
  35. (2008). Molecular identi cation and expression of heat shock cognate (HSC70) in the paci c white shrimp Litopenaeus vannamei. doi
  36. (1996). Mutational analysis of the hsp70-interacting protein Hip.
  37. (2006). Occurrence and recent long-distance dispersal of deep-sea hydrothermal vent shrimps. doi
  38. (2008). Phylogeny of Decapoda using two nuclear protein-coding genes: origin and evolution of the Reptantia. doi
  39. (2005). Quantitative mRNA expression pro ling of heat-shock protein families in rainbow trout cells. doi
  40. (1993). Stress proteins in aquatic organisms: an environmental perspective. doi
  41. (1998). Temperature and sul de tolerance of hydrothermal vent fauna.
  42. (2006). Temperature resistance studies on the deep-sea vent shrimp Mirocaris fortunata. doi
  43. (1992). The biology of hydrothermal vent animals: physiology, biochemistry, and autotrophic symbioses.
  44. (1956). The effects of salinity changes on the respiratory rate of the prawn Palaemonetes varians (Leach). doi
  45. (2003). The evolutionary and ecological role of heat shock proteins. doi
  46. (1993). The function of heat-shock proteins in stress tolerance: degradation and reactivation of damaged proteins. doi
  47. (1988). The heat-shock proteins. doi
  48. (1964). The moulting behaviour of Palaemonetes varians doi
  49. (2009). The ocean is not deep enough: pressure tolerances during early ontogeny of the blue mussel Mytilus edulis. doi
  50. (2008). Thermal biology of the deep-sea vent annelid Paralvinella grasslei: in vivo studies. doi
  51. (2005). Thermal history-dependent expression of the hsp70 gene in purple sea urchins: biogeographic patterns and the effect of temperature acclimation. doi
  52. (2000). Time course and magnitude of synthesis of heat-shock proteins in congeneric marine snails (Genus Tegula) from different tidal heights. doi
  53. (1999). Trophic ecology of Rimicaris exoculata: a combined lipid abundance/stable isotope approach. doi
  54. (1986). Two new Caridean shrimps (Bresillidae) from a hydrothermal eld on the Mid-Atlantic Ridge. doi

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