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Using small molecules to facilitate exchange of bicarbonate and chloride anions across liposomal membranes

By Jeffery T. Davis, Philip A. Gale, Oluyomi A. Okunola, Pilar Prados, Jose Carlos Iglesias-Sánchez, Tomás Torroba and Roberto Quesada


Bicarbonate is involved in a wide range of biological processes, which include respiration, regulation of intracellular pH and fertilization. In this study we use a combination of NMR spectroscopy and ion-selective electrode techniques to show that the natural product prodigiosin, a tripyrrolic molecule produced by microorganisms such as Streptomyces and Serratia, facilitates chloride/bicarbonate exchange (antiport) across liposomal membranes. Higher concentrations of simple synthetic molecules based on a 4,6-dihydroxyisophthalamide core are also shown to facilitate this antiport process. Although it is well known that proteins regulate Cl-/HCO3- exchange in cells, these results suggest that small molecules may also be able to regulate the concentration of these anions in biological systems

Topics: QD
Year: 2009
OAI identifier:
Provided by: e-Prints Soton

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