Prevalence and resistance of Campylobacter spp. in retail poultry meat

Abstract

Cilj rada bio je ustvrditi prevalenciju bakterija roda Campylobacter u pilećem mesu u maloprodaji grada Rijeke te ispitati nji- hovu osjetljivost na antibiotike. Usporediti dobivene rezultate s učestalošću i profilom otpornosti na antibiotike humanih izolata kampilobaktera. Ukupno 60 uzoraka pilećeg mesa bakteriološki je analizirano standar- diziranim postupcima odvajanja i identifi- kacije za detekciju kampilobaktera u hrani. Ispitana je osjetljivost svih izolata na ampi- cilin, eritromicin, ciprofloksacin, gentamicin i tetracikline. Rezultati su uspoređeni s hu- manim izolatima kampilobaktera izdvoje- nih u istom periodu iz 6444 uzoraka stolice bolesnika s akutnom dijarealnom bolešću. U pilećem mesu prevalencija kampilobaktera iznosi 31,7 %, a u ljudi 4,2 %. Prevladavao jeC.jejuni(78,9%umesuperadii89%u stolici bolesnika). Svi izolati bili su osjetljivi na gentamicin. Oko četvrtina izolata iz mesa peradi bila je otporna na eritromicin, dok su humani izolati bili osjetljivi. 33 % C. jejuni iz peradi, a 55 % humanih izolata bilo je otpor- no na ciprofloksacin, dok je 53 % C. jejuni iz mesa peradi za razliku od 9 % humanih izo- lata bilo otporno na tetracikline. U pilećem mesu u maloprodaji prisutne su bakterije roda Campylobacter koje predstavljaju zna- čajan rizik u za pojavu kampilobakterioze u ljudi. Rezultati ispitivanja osjetljivosti na antibiotike ovih bakterija upućuju na važ- nost ograničavanja uporabe antimikrobnih sredstava kao promotora rasta u peradi kako bi se spriječio rast bakterijske rezistencije i prijenos rezistencije s izolata iz pilećeg mesa na sojeve kampilobaktera koji izazivaju obo- ljenje u ljudi putem hrandbenog lanca.The objective of study was to determine the prevalence of Campylobacter spp. in retail poultry meat in Rijeka and to investigate bacterial susceptibility to antibiotics. The goal was to associate resistance to certain antimicrobial agents of poultry meat isolates with the resistance to antibiotics of human isolates. During 2011, a total of 60 retail chickens of various suppliers in Rijeka were collected. Bacteriological analyzes have been carried out using standard methods of separation and identification for the detection of campylobacters in food. At the same time 6444 stool samples from diarrhoeal patients were examined for the presence of campylobacters. The sensitivity of all isolates to ampicillin, erythromycin, ciprofloxacin, gentamicin and tetracycline was investigated. Campylobacters were detected in 31.7% poultry samples and in 4.2% human stools. C. jejuni was dominant species (78.9% of poultry isolates and 89% of human isolates). All isolates were sensitive to gentamicin. Approximately 25% of poultry meat isolates were resistant to erythromycin, while human isolates were susceptible. 33% of C. jejuni from poultry and 55% of human isolates were resistant to ciprofloxacin whereas 53% C. jejuni from poultry meat in contrast to 9 % of human isolates were resistant to tetracycline. In retail poultry meat, Campylobacter spp. are present which pose a significant risk for the occurrence of campylobacterosis in humans. The results of antibiotic susceptibility tests indicate the importance of limiting the use of antimicrobial agents as growth promoters in poultry to prevent the growth of bacterial resistance and its potential transfer from chicken meat isolates to human strains through the food chain

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