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Tyrosine phosphorylation of myosin heavy chain during skeletal muscle differentiation: an integrated bioinformatics approach

By D.F. Harney, R. Butler and Richard J. Edwards

Abstract

Background: Previously it has been shown that insulin-mediated tyrosine phosphorylation of myosin heavy chain is concomitant with enhanced association of C-terminal SRC kinase during skeletal muscle differentiation. We sought to identify putative site(s) for this phosphorylation event.<br/><br/>Results: A combined bioinformatics approach of motif prediction and evolutionary and structural analyses identified tyrosines163 and 1856 of the skeletal muscle heavy chain as the leading candidate for the sites of insulin-mediated tyrosine phosphorylation.<br/><br/>Conclusion: Our work is suggestive that tyrosine phosphorylation of myosin heavy chain, whether in skeletal muscle or in platelets, is a significant event that may initiate cytoskeletal reorganization of muscle cells and platelets. Our studies provide a good starting point for further functional analysis of MHC phosphor-signalling events within different cells.<br/

Year: 2005
OAI identifier: oai:eprints.soton.ac.uk:151151
Provided by: e-Prints Soton

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Citations

  1. (1996). A gene map of the human genome. Science doi
  2. (1995). Alitalo K: Overexpressed Csk tyrosine kinase is localized in focal adhesions, causes reorganization of alpha v beta 5 integrin, and interferes with HeLa cell spreading. Mol Cell Biol
  3. (2001). AR: The tyrosine kinase c-Src is required for 1,25(OH)2-vitamin D3 signalling to the nucleus in muscle cells. Biochim Biophys Acta doi
  4. (2003). Birney E: Ensembl 2002: accommodating comparative genomics. Nucleic Acids Res
  5. (1999). C-terminal Src kinase associates with ligand-stimulated insulin-like growth factor-I receptor. doi
  6. (1994). Cantley LC: Use of an oriented peptide library to determine the optimal substrates of protein kinases. doi
  7. (2001). Chuang LM: Cloning, mapping, and characterization of the human sorbin and SH3 domain containing 1 (SORBS1) gene: a protein associated with c-Abl during insulin signaling in the hepatoma cell line Hep3B. Genomics doi
  8. (2001). Chuang LM: Molecular scanning of the human sorbin and SH3-domain-containing-1 (SORBS1) gene: positive association of the T228A polymorphism with obesity and type 2 diabetes. Hum Mol Genet doi
  9. (2000). Coevolution of head, neck, and tail domains of myosin heavy chains. doi
  10. (1983). Dictionary of protein secondary structure: pattern recognition of hydrogen-bonded and geometrical features. Biopolymers doi
  11. (2000). DT: The PSIPRED protein structure prediction server. Bioinformatics doi
  12. (2000). Flexible sequence similarity searching with the FASTA3 program package. Methods Mol Biol doi
  13. (1997). GeneCards: encyclopedia for genes, proteins and diseases. doi
  14. (2004). HAQESAC: Homologue Alignment Quality, Establishment of Subfamilies and Ancestor Construction.
  15. (2000). Identification and analysis of the myosin superfamily in Drosophila: a database approach.
  16. (1986). Isolation of circumferential microtubules from platelets without simultaneous fixation. Blood
  17. (1995). Kinzler KW: Serial analysis of gene expression. Science doi
  18. (1996). Lipman DJ: Basic local alignment search tool. doi
  19. (2003). MB: Scansite 2.0: Proteome-wide prediction of cell signaling interactions using short sequence motifs. Nucleic Acids Res doi
  20. (1995). Milner-White EJ: RASMOL: biomolecular graphics for all. Trends Biochem Sci doi
  21. Myosins (Protein Profile S) doi
  22. (2003). PB: Proteomic analysis of platelets detects the May-Hegglin gene, non-muscle myosin heavy chain A in platelets and demonstrates its tyrosine phosphorylation following thrombin stimulation. [abstract]. Circulation
  23. (2000). PE: The Protein Data Bank. Nucleic Acids Res doi
  24. (1983). Platelet and megakaryocyte shape change: triggered alterations in the cytoskeleton. Semin Hematol
  25. (1983). Rao GH: Influence of a microtubule stabilizing agent on platelet structural physiology.
  26. (1998). Saltiel AR: A novel, multifuntional c-Cbl binding protein in insulin receptor signaling in 3T3-L1 adipocytes. Mol Cell Biol
  27. (1998). Saltiel AR: A role for CAP, a novel, multifunctional Src homology 3 domain-containing protein in formation of actin stress fibers and focal adhesions. doi
  28. (2002). Saltiel AR: CAP defines a second signalling pathway required for insulin-stimulated glucose transport. Nature
  29. (1999). Sequence and structure-based prediction of eukaryotic protein phosphorylation sites. doi
  30. (1988). Sharp PM: CLUSTAL: a package for performing multiple sequence alignment on a microcomputer. Gene doi
  31. (2002). Tavare JM: Reciprocal feedback regulation of insulin receptor and insulin receptor substrate tyrosine phosphorylation by phosphoinositide 3-kinase in primary adipocytes. doi
  32. (1998). The IRS-signaling system: a network of docking proteins that mediate insulin and cytokine action. Recent Prog Horm Res doi
  33. (2003). The SWISS-PROT protein knowledgebase and its supplement TrEMBL in 2003. Nucleic Acids Res
  34. (1994). Using the FASTA program to search protein and DNA sequence databases. Methods Mol Biol doi

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