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Design and development of a site specific protein patterning technique for use in a microfluidic antibody separation device

By Chrisopher W.A. Johnson


The rapid quantification of the concentration of different immunoglobulins classes from patient serum is required to diagnose patients in the early stages of sepsis. Microfluidic point of care technology can improve diagnostics by decreasing the analysis time, and integrating parallel analysis in a single portable device. The design of a novel method to fabricate surfaces presenting multiple micron scale protein motifs, for integration within a microfluidic channel device, is described in this thesis. <br/><br/>Initial research focussed on conjugating protein motifs on silicon &lt;100&gt; substrates in micron and submicron scale patterns. A method involving the UV-initiated conjugation of a heterobifunctional linker, undecylenic acid N-Hydroxysuccinimde ester (UANHS), to a hydrogen terminated silicon surface was investigated. A photolithographic mask and phase mask were used to form micron and submicron UANHS motifs respectively, on silicon. The conjugation of protein with UANHS motifs was investigated to determine how reproducible the patterns were. The conjugation of streptavidin, streptavidin-FITC, NeutrAvidin, single domain protein L and multidomain protein L to silicon surfaces, upon reaction with UANHS, were investigated. Fluorescently labelled probes that associated with the protein motifs were used to confirm successful conjugation of protein to the silicon. Micron scale motifs of streptavidin, streptavidin-FITC, NeutrAvidin and single domain protein L could be formed reproducibly on silicon. Using a phase mask 140 nm motifs of streptavidin-FITC, conjugated to silicon, were achieved. <br/><br/>Also an alternative method to pattern multiple proteins onto glass surfaces was investigated. A 500,000 MW dextran was modified to incorporate an aryl azide moiety, which was subsequently immobilised on glass surfaces. A method to synthesise and characterise the aryl azide conjugated dextran was investigated, as well as methods to characterise and improve the reproducibility of the aryl azide conjugated dextran layer immobilised on the glass surface. Two photolithographic masks and glass surfaces with alignment marks were fabricated. The masks were used to form micron scale protein motifs, via a photoinitiated conjugation reaction, on the aryl azide conjugated dextran surface. An in-house alignment system was built and a method to produce adjacent protein motifs was investigated. Two adjacent micron scale patterns of multidomain protein L and protein A were achieved. The surface density of conjugated protein L was investigated and a density of ~1.16x10<sup>11</sup> molecules/cm<sup>2</sup> was confirmed. This approach offers a method to attach high density micron scale protein motifs, aligned with micron scale resolution, which is vital to the realisation of a microfluidic point of care device

Topics: QA75, QR180
Year: 2010
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Provided by: e-Prints Soton
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  1. (2007). A
  2. (1985). A and Conde F P
  3. (2007). A and Cooper A
  4. (1999). A and Ligler F S
  5. (1991). A and Shortle D
  6. (2007). A biomolecule friendly photolithographic process for fabrication of protein microarrays on polymeric films coated on silicon chips
  7. (2008). A F
  8. (2001). A factorial analysis of silanization conditions for the immobilization of oligonucleotides on glass surfaces
  9. (1990). A General Method for Highly Selective Cross-Linking of Unprotected Polypeptides via pH-Controlled Modification of NTerminal alpha-Amino
  10. (2008). A micromachined elastomeric tip array for contact printing with variable dot size and density
  11. (1985). A non-immune interaction between the light chain of human immunoglobulin and a surface component of a Peptococcus magnus strain
  12. (1990). A Novel Hydrogel Matrix on Gold Surfaces in SurfacePlasmon Resonance Sensors for Fast and Efficient Covalent Immobilization of Ligands
  13. (1996). A novel organic solvent-based coupling method for the preparation of covalently immobilized proteins on gold
  14. (2007). A parylene lift-off process with microfluidic channels for selective protein patterning
  15. (2008). A photochemical method for immobilization of azidated dextran onto aminated poly(ethylene terephthalate) surfaces
  16. (2004). A photochemical method for patterning the immobilization of ligands and cells to self-assembled monolayers
  17. (2004). A simple approach to micropatterning and surface modification of poly(dimethylsiloxane)
  18. (2006). A study on photolinkers used for biomolecule attachment to polymer surfaces Analytical and
  19. (2009). A T and Goyal A
  20. (1940). A Type-Specific Antigenic Protein Derived from the Staphylococcus The
  21. (2007). A W and Delaney A
  22. (2005). Accelerated lymphocyte death in sepsis occurs by both the death receptor and mitochondrial pathways
  23. (2007). Affinity chromatographic purification of antibodies
  24. (2003). Aging effects on oxidized and amine-modified poly(dimethylsiloxane) surfaces studied with chemical forced titrations: Effects on electroosmotic flow rate in microfluidic channels
  25. (1993). Alkyl Monolayers Covalently Bonded to Silicon Surfaces
  26. (2001). Aminoterminated organic monolayers on hydrogen-terminated silicon surfaces
  27. (1992). and Bengtsson A
  28. (2007). Anion chelation by amido acid functionalized fused quartz/water interfaces studied by nonlinear optics
  29. (2003). Antibody microarrays: An evaluation of production parameters
  30. (2009). Application of Physicochemically Modified Silicon Substrates as Reverse-Phase Protein Microarrays
  31. (2006). Application of surface acoustic waves for optimisation of biocompatibility of carboxymethylated dextran surfaces Surface &
  32. (2009). Approach for Reliable Evaluation of Drug Proteins Interactions Using Surface Plasmon Resonance Technology Analytical
  33. (2008). Autoantibodies in lung cancer: possibilities for early detection and subsequent cure
  34. (2009). Bioconjugation techniques for microfluidic biosensors Analytical and
  35. (2006). Biofunctionalisation of porous silicon (PS) surfaces by using homobifunctional cross-linkers
  36. (2004). Biomaterials: Where we have been and where we are going
  37. (2006). Biomicroarray fabrication techniques - A review Critical Reviews in
  38. (2006). Biomolecular linear motors confined to move upon micro-patterns on glass
  39. (2009). Biosensor analyses of serum autoantibodies: application to antiphospholipid syndrome and systemic lupus erythematosus Analytical and
  40. (2002). Carbohydrate arrays for the evaluation of protein binding and enzymatic modification
  41. (2007). Characterization and antitumor activity of a polysaccharide from Strongylocentrotus nudus eggs Carbohydrate Polymers
  42. (2004). Characterization of protein immobilization on alkyl monolayer modified silicon(111) surface Sensors and Actuators B-Chemical
  43. (2000). Characterization of the binding interface between the E-domain of Staphylococcal protein A and an antibody Fv-fragment (vol 39, pg 26,
  44. (1995). Cloning, expression and purification of Ppl-1, a kappa-chain binding protein, based upon protein L from Peptostreptococcus magnus
  45. (2004). Colloidal lithographic nanopatterning via reactive ion etching
  46. (2009). Comparative study of SPR and ELISA methods based on analysis of CD166/ALCAM levels in cancer and control human sera
  47. (2000). Comparison between different strategies of covalent attachment of DNA to glass surfaces to build DNA microarrays
  48. (1981). Comparison of Mechanisms of Interaction between Protein-a from Staphylococcus-Aureus and Human Monoclonal Igg, Iga and Igm
  49. (2007). Comparison of surface and hydrogel-based protein microchips
  50. (2001). Complex between Peptostreptococcus magnus protein L and a human antibody reveals structural convergence in the interaction modes of Fab binding proteins
  51. (2001). Complex between Peptostreptococcus magnus protein L and a human antibody reveals structural convergence in the interaction modes of Fab binding proteins Structure (Camb)
  52. (2008). Continuous-flow microfluidic printing of proteins for array-based applications including surface plasmon resonance imaging
  53. (2009). Continuous, Real-Time Monitoring of Cocaine in Undiluted Blood Serum via a Microfluidic, Electrochemical Aptamer-Based Sensor
  54. (2004). Contributions of amino acid side chains to the kinetics and thermodynamics of the bivalent binding of protein L to Ig K light chain
  55. (2008). Covalent immobilization of antibody fragments on well-defined polymer brushes via site-directed method Colloids and Surfaces B-Biointerfaces
  56. (2002). Covalent immobilization of chitosan/heparin complex with a photosensitive hetero-bifunctional crosslinking reagent on
  57. (1983). Cross-Linking of Protein by Omega-Maleimido Alkanoyl N-Hydroxysuccinimido
  58. (2008). Crosslinking and stabilization of high fractional free volume polymers for gas separation
  59. (2000). Crystal structure of a Staphylococcus aureus protein A domain complexed with the Fab fragment of a human IgM antibody: Structural basis for recognition of B-cell receptors and superantigen activity
  60. (1981). Crystallographic Refinement and Atomic Models of a Human Fc Fragment and Its
  61. (2008). Current development in microfluidic immunosensing chip
  62. (2008). del Campo A
  63. (2008). Delivery of human fibroblast cells by piezoelectric drop-on-demand inkjet printing
  64. (2002). Determination of structural peculiarities of dexran, pullulan and gamma-irradiated pullulan by Fourier-transform
  65. (2008). Development of a digital microfluidic platform for point of care testing Lab on a
  66. (2003). Development of autoantibodies before the clinical onset of systemic lupus erythematosus New England
  67. (2008). Direct delivery and submicrometer Patterning of DNA by a nanofountain probe
  68. (2004). Direct force measurement of the stability of poly(ethylene glycol)-polyethylenimine graft films
  69. (2007). Directed formation of micro- and nanoscale patterns of functional light-harvesting LH2 complexes
  70. (2005). DNA single strands tethered to fused quartz/water interfaces studied by second harmonic generation
  71. (2004). DNA-directed protein immobilization on mixed self-assembled monolayers via a Streptavidin bridge
  72. (2005). Early and innovative interventions for severe sepsis and septic shock: taking advantage of a window of opportunity
  73. (2001). Early goal-directed therapy in the treatment of severe sepsis and septic shock The New England journal of medicine
  74. (2002). Elastic backscattering of electrons: determination of physical parameters of electron transport processes by elastic peak electron spectroscopy
  75. (1993). Electrophilic Siloxane-Based Self-Assembled Monolayers for Thiol-Mediated Anchoring of Peptides and
  76. (2004). Electrostatic interactions in protein adsorption probed by comparing lysozyme and succinylated lysozyme Colloids and Surfaces B-Biointerfaces
  77. (2005). Emerging technologies in
  78. (1993). Engel A
  79. (2005). Engineering silicon oxide surfaces using self-assembled monolayers
  80. (1983). Enhanced Survival in Pseudomonas-Aeruginosa Septicemia Associated with HighLevels of Circulating Antibody to Escherichia-Coli Endotoxin
  81. (2006). Enhancement of protein adsorption induced by surface roughness
  82. (1998). Enzymatic ligation reactions of DNA &quot;words&quot; on surfaces for DNA computing
  83. (2007). Evaluation of surface chemistries for antibody microarrays
  84. (2006). F F and Majumdar A
  85. (2007). Fabrication of integrated patterns using lithography and particles assembling techniques
  86. (2007). Fabrication of long-term hydrophilic surfaces of poly(dimethyl siloxane) using 2-hydroxy ethyl methacrylate Sensors and Actuators BChemical
  87. (2003). Fabrication of patterned multicomponent protein gradients and gradient arrays using microfluidic depletion
  88. (2009). Fabrication of protein chips based on 3-aminopropyltriethoxysilane as a monolayer
  89. (2010). Fabrication of versatile channel flow cells for quantitative electroanalysis using prototyping
  90. (2009). Flow-through functionalized PDMS microfluidic channels with dextran derivative for ELISAs Lab on a
  91. (1998). Formation of adsorbed protein layers
  92. (1976). Formation of Extracellular Protein-a by Staphylococcus-Aureus
  93. (2008). From particle self-assembly to functionalized sub-micron protein patterns
  94. (2007). Funatsu T and Ohdomari I
  95. (1996). Functionalisation of Si/SiO2 and glass surfaces with ultrathin dextran films and deposition of lipid bilayers
  96. (2001). Global analysis of protein activities using proteome chips
  97. (2009). Grafting of protein L-binding activity onto recombinant antibody fragments
  98. (2005). Hauser A E, Hiepe F and Radbruch A
  99. (1992). High Resolution XPS of Organic Polymers
  100. (2009). High-density protein patterning through selective plasma-induced fluorocarbon deposition on Si substrates
  101. (2008). High-performance immunoassays based on through-stencil patterned antibodies and capillary systems
  102. (2000). High-quality alkyl monolayers on silicon surfaces
  103. (1998). Highly stable Si-C linked functionalized monolayers on the silicon (100) surface
  104. (1991). Human-Iga and Igg F(Ab')2 That Bind to Staphylococcal Protein-a Belong to the Vhiii-Subgroup
  105. (2003). Immobilization and surface characterization of NeutrAvidin biotin-binding protein on different hydrogel interlayers
  106. (2004). Immobilization of erythropoietin to culture erythropoietin-dependent human leukemia cell line
  107. (2002). Immobilization of homooligonucleotide probe layers onto Si/SiO2 substrates: characterization by electrochemical impedance measurements and radiolabelling
  108. (2004). Immobilization of oriented protein molecules on poly(ethylene glycol)-coated
  109. (1991). Immobilization of Proteins to a Carboxymethyldextran-Modified Gold Surface for Biospecific Interaction Analysis in Surface-Plasmon Resonance Sensors Analytical Biochemistry
  110. (2003). Immune suppression and isolated severe head injury: a significant clinical problem
  111. (2005). Immunobiology : the immune system in health and disease
  112. (1993). Immunoglobulin Binding Specificities of the Homology Regions (Domains)
  113. (2002). Immunoglobulin transport across polarized epithelial cells
  114. (1997). Immunological responses to thermal injury
  115. (2002). Improvements to the enzyme-developed radial immunodiffusion technique
  116. (2004). Improving the intein-mediated, sitespecific protein biotinylation strategies both in vitro and in vivo
  117. (2002). Incorporation of azides into recombinant proteins for chemoselective modification by the Staudinger ligation
  118. (1996). Increased plasma IgE levels in patients with sepsis after traumatic injury
  119. (2008). Integrated barcode chips for rapid, multiplexed analysis of proteins in microliter quantities of blood
  120. (2008). Integrated direct DNA/protein patterning and microfabrication by focused ion beam milling
  121. (1999). Interactions between a single immunoglobulin-binding domain of protein L from Peptostreptococcus magnus and a human kappa light chain The Biochemical journal 340
  122. (2008). Interfacial adsorption and denaturization of human milk and recombinant rice lactoferrin
  123. (2006). Intravenous polyclonal IgMenriched immunoglobulin therapy in sepsis: a review of clinical efficacy in relation to microbiological aetiology and severity of sepsis
  124. (2007). K and Hillier A C
  125. (2007). Khademhosseini A
  126. (2006). Kinetics, spectroscopy, and computational chemistry of arylnitrenes
  127. (2006). Koh A E and Chen W
  128. (2005). Label-free detection of smallmolecule-protein interactions by using nanowire nanosensors
  129. (2007). Large-area protein nano-arrays patterned by soft lithography
  130. (2006). Laserinduced forward transfer of liquids: Study of the droplet ejection process
  131. (2002). Long-range selfassembly of a polyunsaturated linear organosilane at the n-Tetradecane/Au(111) interface studied by
  132. (2006). Low melting point agarose as a protection layer in photolithographic patterning of aligned binary proteins Lab on a
  133. (2006). Management of sepsis during the early &quot;golden hours&quot;
  134. (2006). Mass transport and surface reactions in microfluidic systems
  135. (2003). Medical progress: The pathophysiology and treatment of sepsis.
  136. (2003). Mencaglia A
  137. (1995). Methods for Site Controlled Coupling to Carboxymethyldextran Surfaces in SurfacePlasmon Resonance Sensors Biosensors &
  138. (2004). Micro patterning of active proteins with perforated
  139. (2006). Micro- and nanofabrication methods in nanotechnological medical and pharmaceutical devices
  140. (2007). Micro/nanopatterning of proteins via contact printing using high aspect ratio PMMA stamps and NanoImprint apparatus
  141. (2006). Microarray technology: beyond transcript profiling and genotype analysis
  142. (2000). Microcontact printing of proteins
  143. (2007). Microcontact printing: A tool to pattern
  144. (2006). Microfabricated &quot;Biomolecular Ink Cartridges&quot; - Surface patterning tools (SPTs) for the printing of multiplexed biomolecular arrays Sensors and Actuators B-Chemical
  145. (2009). Microfluidic chip for combinatorial mixing and screening of assays Lab on a
  146. (2005). Microfluidic devices for environmental monitoring TracTrends in
  147. (2008). Microfluidic immunoaffinity separations for bioanalysis
  148. (2007). Microfluidic immunoassays as rapid saliva-based clinical diagnostics
  149. (1998). Microfluidic networks for chemical patterning of substrate: Design and application to bioassays
  150. (2008). Microfluidic protein patterning on silicon nitride using solvent-extracted poly(dimethylsiloxane) channels Sensors and Actuators BChemical
  151. (2003). Microfluidic systems in proteomics
  152. (2008). Microfluidics closes in on point-of-care assays
  153. (2008). Microfluidics for drug discovery and development: From target selection to product lifecycle management Drug Discovery Today
  154. (2005). Microfluidics for processing surfaces and miniaturizing biological assays
  155. (2007). Micrometer and nanometer scale photopatterning of selfassembled monolayers of phosphonic acids on aluminum oxide
  156. (2005). Micropatterning of biomolecules on glass surfaces modified with various functional groups using photoactivatable biotin
  157. (2006). Mixed azideterminated monolayers: A platform for modifying electrode surfaces
  158. (1995). Model for the Complex between Protein-G and an Antibody Fc Fragment
  159. (2008). Modified fabrication process of protein chips using a shortchain self-assembled monolayer
  160. (2007). Molecular mechanisms of antibody somatic hypermutation Annual Review of
  161. (2004). Monitoring of recombinant survival motor neuron protein using fiber-optic surface plasmon resonance
  162. (2007). Muck A and Svatos A
  163. (2006). Multi-colour micro-contact printing based on microfluidic network inking
  164. (2008). Multifunctional surfaces with discrete functionalized regions for biological applications
  165. (2007). Nanocarriers as an emerging platform for cancer therapy
  166. (2003). Nanocontact printing: A route to sub50-nm-scale chemical and biological patterning
  167. (2003). Nanometer-scale roughness having little effect on the amount or structure of adsorbed protein
  168. (2006). Nanopatterning proteins and peptides
  169. (2007). Native protein nanolithography that can write, read and erase
  170. (1997). NMR analysis of the interaction between protein L and Ig light chains
  171. (1998). NMR study of the interaction between the B domain of staphylococcal protein A and the Fc portion of immunoglobulin
  172. (2004). Observation and characterization of the interaction between a single immunoglobulin binding domain of protein L and two equivalents of human kappa light chains The Journal of biological chemistry
  173. (2007). Observation of nanostructure by scanning near-field optical microscope with small sphere probe
  174. (2009). Oligo(ethylene oxide) Self-Assembled Monolayers, with Self-Limiting Packing Densities for the Inhibition of Nonspecific Protein
  175. (1993). On the interaction between protein L and immunoglobulins of various mammalian species Scandinavian journal of immunology
  176. (2007). On-chip micropatterning of plastic (cylic olefin copolymer, COC) microfluidic channels for the fabrication of biomolecule microarrays using photografting methods
  177. (2008). One-step photochemical attachment of NHS-terminated monolayers onto silicon surfaces and subsequent functionalization
  178. (2007). One-step photochemical introduction of nanopatterned protein-binding functionalities to oligo(ethylene glycol)-terminated self-assembled monolayers
  179. (2008). Optimisation of analyte transport in integrated microfluidic affinity sensors for the quantification of low levels of analyte Sensors and Actuators B-Chemical
  180. (2003). Optimizing antibody immobilization strategies for the construction of protein microarrays
  181. (2005). Organic azides: An exploding diversity of a unique class of compounds
  182. (2002). Organometallic chemistry on silicon and germanium surfaces
  183. (2008). Pachot A and Lepape A
  184. (2007). Patterned biofunctional poly(acrylic acid) brushes on silicon surfaces
  185. (1997). Patterned delivery of immunoglobulins to surfaces using microfluidic networks
  186. (2004). Patterning enzymes inside microfluidic channels via photoattachment chemistry
  187. (2004). Patterning of DNA nanostructures on silicon surface by electron beam lithography of self-assembled monolayer
  188. (2006). Persisting low monocyte human leukocyte antigen-DR expression predicts mortality in septic shock Intensive Care
  189. (2004). Photochemistry of fluorophenyl azides in aniline asymmetric fluoroazobenzenes by N-H singlet nitrene insertion
  190. (1998). Photochemistry of fluorophenyl azides in diethylamine. Nitrene reaction versus ring expansion
  191. (2000). Photoreactivity of unsaturated compounds with hydrogen-terminated silicon(111)
  192. (1994). Photosensitive Self-Assembled Monolayers on Gold -
  193. (2002). Physics and applications of microfluidics in biology
  194. (2007). Plasma assisted production of chemical nano-patterns by nano-sphere lithography: application to biointerfaces
  195. (2009). Point-of-care testing of proteins
  196. (1999). Polymer brushes that resist adsorption of model proteins:
  197. (2008). Polymer microfabrication technologies for microfluidic systems
  198. (2009). Positioning Multiple Proteins at the Nanoscale with Electron Beam Cross-Linked Functional Polymers
  199. (2004). Preparation and photoinduced patterning of azidoformate-terminated self-assembled monolayers
  200. (2009). Preparation of protein microarrays on non-fouling and hydrated poly(ethylene glycol) hydrogel substrates using photochemical surface modification
  201. (2007). Prevention of immune cell apoptosis as potential therapeutic strategy for severe infections Emerging Infectious
  202. (2005). Prevention of lymphocyte apoptosis -A potential treatment of sepsis?
  203. (2007). Primary versus ternary adsorption of proteins onto
  204. (2005). Printing of protein microarrays via a capillary-free fluid jetting mechanism
  205. (2000). Printing proteins as microarrays for high-throughput function determination
  206. (2007). Protein A chromatography for antibody purification
  207. (2002). Protein adsorption on model surfaces with controlled nanotopography and chemistry
  208. (2007). Protein and cell micropatterning and its integration with micro/nanoparticles assembly
  209. (2007). Protein immobilization strategies for protein biochips
  210. (1992). Protein L from Peptostreptococcus magnus binds to the kappa light chain variable domain The Journal of biological chemistry
  211. (1990). Protein L, a bacterial immunoglobulin-binding protein and possible virulence determinant
  212. (1989). Protein L: an immunoglobulin light chain-binding bacterial protein. Characterization of binding and physicochemical properties
  213. (1988). Protein L. A novel bacterial cell wall protein with affinity for Ig L chains
  214. (1997). Protein retention in hydrophobic interaction chromatography: Modeling variation with buffer ionic strength and column hydrophobicity
  215. (2004). Protein surface patterning using nanoscale PEG hydrogels
  216. (2005). Protein-detecting microarrays: Current accomplishments and requirements
  217. (1985). Protein-G - a Powerful Tool for Binding and Detection of Monoclonal and Polyclonal Antibodies
  218. (2004). Protein-resistant monolayers prepared by hydrosilylation of alpha-oligo(ethylene glycol)-omega-alkenes on hydrogenterminated silicon (111) surfaces
  219. (2008). Protein-resistant poly(ethylene oxide)-grafted surfaces: Chain density-dependent multiple mechanisms of action
  220. (2003). ProteoChip: A highly sensitive protein microarray prepared by a novel method of protein, immobilization for application of protein-protein interaction studies
  221. (1993). Proton nuclear magnetic resonance sequential assignments and secondary structure of an immunoglobulin light chain-binding domain of protein
  222. (1984). Purification and Some Properties of Streptococcal ProteinG, Protein-a Novel Igg-Binding Reagent
  223. (2009). Quantification of DNA and protein adsorption by optical phase shift
  224. (2006). Quantifying specific antibody concentrations by enzyme-linked immunosorbent assay using slope correction Clinical and Vaccine
  225. (2006). Quartz crystal microbalance immunosensors for environmental monitoring Biosensors
  226. (1978). Rapid Photolytic Release of Adenosine 5'-Triphosphate from a Protected Analog - Utilization by Na-K Pump of Human Red
  227. (2009). Rapid prototyping of microfluidic systems using a PDMS/polymer tape composite Lab on a
  228. (2009). Reaction of Glycidyl Methacrylate at the Hydroxyl and Carboxylic Groups of Poly(vinyl alcohol) and Poly(acrylic acid): Is This Reaction Mechanism Still Unclear?
  229. (2000). Real-time immunoassay of antibody activity in serum by surface plasmon resonance biosensor
  230. (1984). Region-X, the Cell-Wall-Attachment Part of Staphylococcal Protein-A
  231. (2007). Registration accuracy in multilevel soft lithography
  232. (2005). Regulation of blood coagulation by the protein C anticoagulant pathway: novel insights into structure-function relationships and molecular recognition Arteriosclerosis, thrombosis, and vascular biology
  233. (2009). Route to Smooth Silica-Based Surfaces Decorated with Novel Self-Assembled Monolayers (SAMs) Containing GlycidylTerminated Very Long Hydrocarbon Chains
  234. (2006). Scanning near-field photolithography-surface photochemistry with nanoscale spatial resolution
  235. (2009). Selection of imprinted nanoparticles by affinity chromatography
  236. (2006). Self-aligned immobilization of proteins utilizing
  237. (2002). Self-assembled monolayers of organosilicon hydrides supported on titanium, zirconium, and hafnium dioxides
  238. (2004). Self-assembled monolayers with different terminating groups as model substrates for cell adhesion studies
  239. (2007). Self-supporting hydrogel stamps for the microcontact printing of proteins
  240. (2007). Sepsis: definition, epiderhiology, and diagnosis
  241. (2008). Sepsis: rethinking the approach to clinical research
  242. (2005). Severe sepsis and therapy with activated protein
  243. (2009). Shedding light on surfacesusing photons to transform and pattern material surfaces
  244. (1991). Silanation of Silica Surfaces - a New Method of Constructing Pure or
  245. (2008). Silane-dextran chemistry on lateral flow polymer chips for immunoassays Lab on a
  246. (2003). Singlet oxygen-mediated damage to proteins and its consequences
  247. (2006). Solution-phase surface modification in intact poly(dimethylsiloxane) microfluidic channels
  248. (2009). Stimuli-responsive interfaces and systems for the control of protein-surface and cell-surface interactions
  249. (2008). Stimuli-responsive surfaces for bio-applications
  250. (2004). Strategies for immobilization of biomolecules in a microarray
  251. (2004). Structural characterization of organic multilayers on silicon(111) formed by immobilization of molecular films on functionalized Si-C linked monolayers
  252. (1992). Structure of peptostreptococcal protein L and identification of a repeated immunoglobulin light chain-binding domain
  253. (2001). Studies on a single immunoglobulin-binding domain of protein L from Peptostreptococcus magnus: the role of tyrosine-53 in the reaction with human
  254. (2007). Study of the laser-induced forward transfer of liquids for laser bioprinting
  255. (2006). Submicron streptavidin patterns for protein assembly
  256. (2005). Surface characterization of 3-glycidoxypropyltrimethoxysilane films on silicon-based substrates Analytical and
  257. (2009). Surface immobilisation of antibody on cyclic olefin copolymer for sandwich immunoassay Biosensors
  258. (2004). Surface modification of ePTFE vascular grafts with O-carboxymethylchitosan Polymer
  259. (2003). Surface modification of poly(dimethylsiloxane) microchannels
  260. (2002). Surface modification of poly(dimethylsiloxane) microfluidic devices by ultraviolet polymer grafting
  261. (2002). Surface modification of Sylgard-184 poly(dimethyl siloxane) networks by ultraviolet and ultraviolet/ozone treatment
  262. (2004). Surface modification of the channels of poly(dimethylsiloxane) microfluidic chips with polyacrylamide for fast electrophoretic separations of proteins
  263. (2000). Surface modification using silanated poly(ethylene glycol)s
  264. (2005). Surface modification with orthogonal photosensitive silanes for sequential chemical lithography and site-selective particle deposition
  265. (2004). Surface plasmon fluorescence immunoassay of free prostate-specific antigen in human plasma at the femtomolar level
  266. (2006). Surface plasmon optical studies of carboxymethyl dextran brushes versus networks
  267. (2006). Surface plasmon resonance-based highly sensitive immunosensing for brain natriuretic peptide using nanobeads for signal amplification
  268. (1999). Surface proteins of gram-positive bacteria and mechanisms of their targeting to the cell wall envelope
  269. (2006). surface to minimize nonspecific adsorption Lab on a
  270. (2005). Surgical trauma and immunosuppression: pathophysiology and potential immunomodulatory approaches Langenbecks Archives of Surgery
  271. (2000). Surveying for surfaces that resist the adsorption of proteins
  272. (2007). Sweet brushes and dirty proteins
  273. (2000). Synthesis and characterization of DNA-modified silicon (111) surfaces
  274. (2005). Synthesis of photoreactive pullulan for surface modification
  275. (2007). Synthesis, patterning and applications of star-shaped poly(ethylene glycol) biofunctionalized surfaces
  276. (2008). Teplyakov A V
  277. (2005). The direct detection of an aryl azide excited state: An ultrafast study of the photochemistry of paraand ortho-biphenyl azide
  278. (2009). The effect of surface microtopography of poly(dimethylsiloxane) on protein adsorption, platelet and cell adhesion Colloids and Surfaces B-Biointerfaces
  279. (1994). The functional units of a peptostreptococcal protein
  280. (2000). The immune system - First of two parts New England
  281. (2002). Thermal hydrosilylation of undecylenic acid with porous silicon
  282. (1997). Thermal/oxidative degradation and stabilization of polyethylene glycol
  283. (2009). Tilting toward immunosuppression
  284. (2007). Transfer printing of DNA by &quot;Click&quot; chemistry
  285. (2001). Trends in miniaturized total analysis systems for point-of-care testing in clinical chemistry Lab on a
  286. (1996). Ultrathin hydrated dextran films grafted on glass: Preparation and characterization of structural, viscous, and elastic properties by quantitative microinterferometry
  287. (2004). Use of IgM and IgA-enriched immunoglobulins in the treatment of severe sepsis and septic shock.
  288. (2007). UV-patterned poly(ethylene glycol) matrix for microarray applications

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