Article thumbnail

DOI:10.2298/ABS1002337P IN VITRO SHOOT REGENERATION FROM SEEDLING EXPLANTS IN BRASSICA VEGETABLES: RED CABBAGE, BROCCOLI, SAVOY CABBAGE AND CAULIFLOWER

By M. Zdravković and D. Vinterhalter

Abstract

Abstract- Brassica oleracea varieties (red cabbage, broccoli, Savoy cabbage and cauliflower) were tested for their ability to regenerate shoots in vitro. Cotyledon, hypocotyl and root explants of 7 day-old seedlings were incubated on Murashige and Skoog’s (MS) medium supplemented with 1 mg l-1 6-benzyladenine (BA) or 6-furfurylaminopurine (KIN) in combination with 0, 0.1, and 0.2 mg l-1 indole-3-butyric acid (IBA). Hypocotyls showed the best explants in almost all varieties tested with a minimum regeneration potential of 75 % and producing 3.5-7.4 shoots per explant. The BA-supplemented media were optimal for both shoot regeneration and multiplication. Shoots rooted maximally (100%) on plant growth regulator-free MS medium containing 2 % or 4 % sucrose. Increased sucrose content improved plant acclimation in the greenhouse

Topics: Brassica oleracea, regeneration, seedling explants, cytokinins, shoot multiplication, plant acclimation UDC 635.33, 581.143
Year: 2014
OAI identifier: oai:CiteSeerX.psu:10.1.1.430.4945
Provided by: CiteSeerX
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • http://citeseerx.ist.psu.edu/v... (external link)
  • http://www.doiserbia.nb.rs/img... (external link)

  • To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.

    Suggested articles