Skip to main content
Article thumbnail
Location of Repository

RESEARCH ARTICLE DEVELOPMENT Protein O-GlcNAcylation Is Required for Fibroblast Growth Factor Signaling in Drosophila

By Kathrin Sauert, Daniel Turnock, Ryan Webster, Daan M. F. Van Aalten, Michael A. J. Ferguson and H. -arno J. Müller

Abstract

Glycosylation is essential for growth factor signaling through N-glycosylation of ligands and receptors and the biosynthesis of proteoglycans as co-receptors. Here, we show that protein O-GlcNAcylation is crucial for fibroblast growth factor (FGF) signaling in Drosophila.Wefoundthatnesthocker (nst) encodes aphosphoacetylglucosaminemutaseandthatnst mutant embryos exhibited low amounts of intracellular uridine 5′-diphosphate–N-acetylglucosamine (UDP-GlcNAc), which disrupted protein O-GlcNAcylation. Nst was required for mitogen-activated protein kinase (MAPK) signaling downstream of FGF but not MAPK signaling activated by epidermal growth factor. nst was dispensable for the function of the FGF ligands and the FGF receptor’s extracellular domain but was essential in the signal-receiving cells downstream of the FGF receptor. We identified the adaptor protein Downstream of FGF receptor (Dof), which interacts with the FGF receptor, as the relevant target for O-GlcNAcylation in the FGF pathway, suggesting that protein O-GlcNAcylation of the activated receptor complex is essential for FGF signal transduction

Year: 2014
OAI identifier: oai:CiteSeerX.psu:10.1.1.417.7542
Provided by: CiteSeerX
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • http://citeseerx.ist.psu.edu/v... (external link)
  • http://davapc1.bioch.dundee.ac... (external link)
  • Suggested articles


    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.