Abstract: In this work, an analytical study to detect the ratio of bilirubin in human body by determining the level of it in blood using a 532nm laser light transported through some samples of bilirubin has been presented. A second harmonic generation of Nd:YAG laser with measured maximum output power 52.5 mW was used to determine the concentration of total bilirubin in blood. Initially, the cuvette was filled with a sample of standard bilirubin level and then it is filled by a sample of blood containing unknown levels of bilirubin. The absorption factor was calculated for four samples of adults ’ blood and five samples of babies ’ blood, and the scattering factor was neglected for each sample. The unknown concentration of total bilirubin was determined and the transmitted power through these samples of blood was measured. In this work, a good matching was obtained between the results of the concentrations of bilirubin in blood using laser technique and the results of the classical medical procedure of measuring the bilirubin concentrations. Therefore, the jaundice in human was detected. I
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