Copyright © American Society for Investigative Pathology Distinct Role of Fibroblast Growth Factor-2 and Vascular Endothelial Growth Factor on Tumor Growth and Angiogenesis

Abstract

Tumors express more than a single angiogenic growth factor. To investigate the relative impact of fibroblast growth factor-2 (FGF-2) and vascular endothelial growth factor (VEGF) on tumor growth and neovascularization, we generated tumor cell transfectants differing for VEGF and/or FGF-2 expression. Human endometrial adenocarcinoma HEC-1-B-derived Tet-FGF-2 cells that express FGF-2 under the control of the tetracycline-responsive promoter (Tet-off system) were further transfected with a VEGF 121 antisense (AS-VEGF) cDNA. Next, Tet-FGF-2 and AS-VEGF/ Tet-FGF-2 cells were transplanted subcutaneously in nude mice that received tetracycline or not in the drinking water. Simultaneous expression of FGF-2 and VEGF in Tet-FGF-2 cells resulted in fast-growin

Download PDF:
_{Sorry, we are unable to provide the full text but you may find it at the following location(s):}

http://citeseerx.ist.psu.edu/v... (external link)

http://www.med.unibs.it/~airc/... (external link)