Backgrounds: Cardiac phantom studies utilized to investigate imaging characteristics for determination of optimal imaging parameters rely on appropriate simulation of the radiotracer biodistribution within the human bodies. A variety of radiotracer concentrations have been used for 99m Tc phantom studies but the methodologies to obtain those values were not clearly mentioned. We introduce and study a count-preserved method to determine the optimal radiotracer concentrations filled in anthropomorphic torso phantom with cardiac insert by utilizing projection data of 99m Tc-MIBI patients. Methods: This study enrolled twenty-three consecutive patients who had normal dipyridamole-stressed 99m Tc-MIBI gated myocardial perfusion SPECT (GSPECT) with LEAP collimation. We summed the planar images of each study and defined ROIs corresponding to the heart, liver and background respectively. The count density of each ROI as (counts/second/pixel) was then calculated. The count densities were adjusted proportionally according to radiotracer doses of 8 mCi at rest and 22 mCi at stress respectively. The anthropomorphic torso phantom with cardiac insert was filled with 99m Tc solution with different concentration levels; 234.58 KBq/ml (6.34 µCi/ml) in the myocardial compartment of the cardiac insert, 351.87 KBq/ml (9.51 µCi/ml) in th
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