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A photoactivatable marker protein for pulse-chase imaging with superresolution

By Jochen Fuchs, Susan Böhme, Franz Oswald, Per Niklas Hedde, Maike Krause, Jörg Wiedenmann and G. Ulrich Nienhaus


IrisFP is a photoactivatable fluorescent protein that combines irreversible photoconversion from a green- to a red-emitting form with reversible photoswitching between a fluorescent and a nonfluorescent state in both forms. Here we introduce a monomeric variant, mIrisFP, and demonstrate how its multiple photoactivation modes can be used for pulse-chase experiments combined with subdiffraction-resolution imaging in living cells by using dual-color photoactivation localization microscopy (PALM)

Year: 2010
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Provided by: e-Prints Soton
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