Siderophore type chelates were detected in nutrient enriched, incubated seawater collected from different biogeographical regions of the Atlantic Ocean. Seawater was enriched with glucose and ammonium, glycine (as a source of carbon and nitrogen) or chitin and ammonium at different concentrations and incubated for up to 3 – 4 days in the dark. Siderophore type chelates were detected using high performance liquid chromatography coupled to inductively coupled plasma mass spectrometry (HPLC-ICP-MS) after complexation with Ga. Samples were subsequently analysed by HPLC - electrospray ionisation mass spectrometry (HPLC<br/>ESI-MS) in order to confirm the identity of the known siderophores, and to obtain the pseudo molecular ions of unknown siderophore type chelates. A total of 22 different siderophore type chelates were resolved in the HPLC-ICP-MS chromatograms. Ten different siderophore type chelates were identified by HPLC-ESI-MS, 3 of which had not previously been identified in nutrient enriched seawater incubations. The concentration and diversity of siderophore typechelates was highest in seawater amended with glucose. The concentrations 1 and diversity of siderophore type chelates also varied with biogeographical area in the Atlantic Ocean, with the North Atlantic Sub-tropical Gyre yielding highest concentrations in incubations, and the South Atlantic Sub-tropical Gyre and Western Tropical Atlantic yielding the highest diversity
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