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By Fingerprinted Contigs

Abstract

considered a reasonable way to order clones due to improved clone libraries and software. BAC libraries provide longer inserts that require fewer clones to cover a region and close gaps versus the shorter inserts with cosmids and do not have the instabilities of the longer YAC clones. Recent sequence ready maps have been constructed with BAC, PACs (P1 Artificial Chromosomes), markers, and global frameworks. Niederfuhr et al. (1998) constructed a sequence ready map of PACs for human chromosome 11p13 by using chromosome walking independently verified by fingerprint analysis. Cao et al. (1999) built a sequence ready map of chromosome 16p13.1-p11.2 by using BACs and previously mapped STSs. Zhu et al. (1999) built a sequence ready map of chromosome 7 of the rice blast fungus Magnaporthe grisea by using BAC contigs assembled by hybridization and integrated with fingerprinted BAC contigs Hoskins et al. (2000) integrated STS content, restriction fingerprinting, and polytene chromosome in situ hybridization to produce a Drosophila melanogaster map for 81 % of the genome. Klein et al. (2000) used amplified fragment length polymorphism (AFLP)based markers integrated with fingerprints to map sorghum. To provide confirmation of overlap and information to merge contigs, the Sanger Centre traditionally has used markers with fingerprints (e.g., see Mungall et al. 1997; Soderlund et al. 1998). An alternative approach by Ding et al. (1999) uses three separate sets of fingerprints to increase the sensitivity of overlap calculation. In the spring of 1999, the Genome Sequencing Center (GSC) in St. Louis started mass-fingerprinting BACs from the RPCI-11 male library constructed a

Year: 2013
OAI identifier: oai:CiteSeerX.psu:10.1.1.371.8128
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