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but enhanced by GTP-gS andAlF4 – (Fig. 2, C and D), indicating that the cytoplasmic domains of b3 and b1 can directly interact with Ga13 and that GTP enhances the interaction. The Ga 13-b 3 interaction was enhanced in platelets adherent to fibrinogen, and by thrombin, which stimulates GTP binding to Ga13 via GPCR (Fig. 2E). Hence, the interaction is regulated by both integrin occupancy and GPCR signaling. To map the b 3 binding site in Ga 13, we incubated cell lysates containing Flag-tagged wild type or truncation mutants of Ga 13 (fig. S5) with GSTb3CD beads. GST-b3CD associated with wild-type Ga 13 and the Ga 13 1 to 212 fragment containing a-helical region and switch region I (SRI), but no

Year: 2010
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