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doi:10.1155/2010/619436 Research Article Investigation on the Interactions of NiCR and NiCR-2H with DNA

By Priyanka Chitranshi, Chang-nan Chen, Patrick R. Jones, Jesika S. Faridi and Liang Xue


Copyright © 2010 Priyanka Chitranshi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. We report here a biophysical and biochemical approach to determine the differences in interactions of NiCR and NiCR-2H with DNA. Our goal is to determine whether such interactions are responsible for the recently observed differences in their cytotoxicity toward MCF-7 cancer cells. Viscosity measurement and fluorescence displacement titration indicated that both NiCR and NiCR-2H bind weakly to duplex DNA in the grooves. The coordination of NiCR-2H with the N-7 of 2 ′-deoxyguanosine 5 ′-monophosphate (5 ′-dGMP) is stronger than that of NiCR as determined by 1 H NMR. NiCR-2H, like NiCR, can selectively oxidize guanines present in distinctive DNA structures (e.g., bulges), and notably, NiCR-2H oxidizes guanines more efficiently than NiCR. In addition, UV and 1 H NMR studies revealed that NiCR is oxidized into NiCR-2H in the presence of KHSO5 at low molar ratios with respect to NiCR (≤4). 1

Year: 2013
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