Abstract Polo-like kinase 1 (Plk1) and Aurora A play key roles in centrosome maturation, spindle assembly, and chromosome segregation during cell division. Here we show that the functions of these kinases during early mitosis are coordinated through Bora, a partner of Aurora A first identified in Drosophila. Depletion of human Bora (hBora) results in spindle defects, accompanied by increased spindle recruitment of Aurora A and its partner TPX2. Conversely, hBora overexpression induces mislocalization of Aurora A and monopolar spindle formation, reminiscent of the phenotype seen in Plk1-depleted cells. Indeed, Plk1 regulates hBora. Following Cdk1-dependent recruitment, Plk1 triggers hBora destruction by phosphorylating a recognition site for SCF " TrCP. Plk1 depletion or inhibition Communicated by: C. Lehner Electronic supplementary material The online version of this article (doi:10.1007/s00412-008-0165-5) contains supplementary material, which is available to authorized users
To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.