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Research Article Chemical Composition and, Cellular Evaluation of the Antioxidant Activity of Desmodium adscendens Leaves

By François Nsemi Mu, Jaouad Bouayed, Abdelouaheb Djilani, Chunyan Yao, Rachid Soulimani and Amadou Dicko

Abstract

Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Desmodium adscendens plant is widely used as juice or tea in various parts of the world against a wide range of diseases. This study determines the quality and the quantity of polyphenols, flavonoids, anthocyanins, and tannins in D. adscendens leaves by UV-spectrophotometry and RP-HPLC methods. In addition, the antioxidant capacity of these phenolic compounds is evaluated by ABTS (2,2 ′-azino-bis(3-ethylbenzothiazoline-6-sulfonic)), DPPH (2,2-diphenyl-1 picrylhydrazyl), and Cellular tests. D. adscendens leaves are mainly composite of flavonoid compounds with 12.8 mg of catechin equivalent (CE)/g dw. The amounts of total polyphenol compounds are 11.1 mg of gallic acid equivalent (GAE)/g dw. The quantity of total anthocyanin and total tannin compounds is not considerable 0.0182 mg CgE/g dw and 0.39 mg CE/g dw, respectively. A direct correlation between phenolic compounds and antioxidant activity is observed (R 2 = 0.96). The RP-HPLC analyses reveal that the main phenolic compound identified in the methanol-water extract is quercetrin dihydrat (2.11 mg/mL). According to the results, it is observed that D. adscendens leaves possess a considerable scavenging antioxidant and antiradical capacity, therefore these antioxidant propertie

Topics: Medicinal pla
Year: 2013
OAI identifier: oai:CiteSeerX.psu:10.1.1.352.232
Provided by: CiteSeerX
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