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Fluorescence quenching of alpha-fetoprotein by gold nanoparticles: effect of dielectric shell on non-radiative decay

By Jian Zhu, Jian-jun Li, A-qing Wang, Yu Chen and Jun-wu Zhao

Abstract

Ó The Author(s) 2010. This article is published with open access at Springerlink.com Abstract Fluorescence quenching spectrometry was applied to study the interactions between gold colloidal nanoparticles and alpha-fetoprotein (AFP). Experimental results show that the gold nanoparticles can quench the fluorescence emission of adsorbed AFP effectively. Furthermore, the intensity of fluorescence emission peak decreases monotonously with the increasing gold nanoparticles content. A mechanism based on surface plasmon resonance–induced non-radiative decay was investigated to illuminate the effect of a dielectric shell on the fluorescence quenching ability of gold nanoparticles. The calculation results show that the increasing dielectric shell thickness may improve the monochromaticity of fluorescence quenching. However, high energy transfer efficiency can be obtained within a wide wavelength band by coating a thinner dielectric shell

Year: 2010
OAI identifier: oai:CiteSeerX.psu:10.1.1.351.966
Provided by: CiteSeerX
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