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Federal Republic of Germany

By Dauter, Howard Terry, Herbert Witzel, Keith and S. Wilson


The structure of 'metal-free ' glucose isomerase of Streptomyces albus strain number YT ATCC 21132 has been analysed and refined at 1-65 A. The space group is/222, with cell dimensions a = 93.9 (1), b = 99.7 (1) and c = 102.9 (1)A, and there is one monomer of the tetrameric molecule per asymmetric unit. The data were recorded from two crystals of the protein using synchrotron radiation from the EMBL beamline X ll at DESY, Hamburg. Data were recorded with an imaging plate scanner designed and built in the EMBL Hamburg outstation. The total data-collection time was less than 12 h and the processing of all data took less than 2 days. The coordinates of the Arthrobacterglucose isomerase refined at a resolution of 2.5A were used as a starting model. The structure of the protein and of 445 associated water molecules in the asymmetric unit were refined by restrained least-squares minimization using all data between 8 and 1.65 A to a final R factor of 1.4.1%

Year: 1990
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