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Metabolism of lithocholic acid in the rat: formation of lithocholic acid 3-0-glucuronide in vivo

By Joanna M. Little, Piotr Zimniak, Karen E. Shattuck, Roger Lester and Anna Radominska


5P-cholanoic) acid were administered by intravenous infusion to rats prepared with a biliary fistula. Analysis of sequential bile samples by thin-layer chromatography (TLC) demonstrated that lithocholic acid glucuronide was present in bile throughout the course of the experiments and that its secretion rate paralleled that of total isotope secretion. Initial confirmation of the identity of this metabolite was obtained by the recovery of labeled lithocholic acid after P-glucuronidase hydrolysis of bile samples. For detailed analysis of biliary metabolites of [ 3H]lithocholic acid, pooled bile samples from infused rats were subjected to reversed-phase chromatography and four major labeled peaks were isolated. After complete deconjugation, the two major compounds in the combined first two peaks were identified as murideoxycholi

Year: 2013
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