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A microsporidian parasite of the genus Spraguea in the nervous tissues of the Japanese anglerfish Lophius litulon

By Mark A. Freeman, Hiroshi Yokoyama and Kazuo Ogawa

Abstract

Abstract. In the present study, a high percentage of Japanese anglerfish, Lophius litulon (Jordan, 1902), contained a microsporidian infection of the nervous tissues. Xenomas were removed and prepared for standard wax histology and transmission electron microscopy (TEM). DNA extractions were performed on parasite spores and used in PCR and sequencing reactions. Fresh spores measured 3.4 × 1.8 µm and were uniform in size with no dimorphism observed. TEM confirmed that only a single developmental cycle and a single spore form were present. Small subunit (SSU) rDNA sequences were>99.5 % similar to those of Spraguea lophii (Doflein, 1898) and Glugea americanus (Takvorian et Cali, 1986) from the European and American Lophius spp. respectively. The microsporidian from the nervous tissue of L. litulon undoubtedly belongs in the genus Spraguea Sprague et Vávra, 1976 and the authors suggest a revision to the generic description of Spraguea to include monomorphic forms and the transfer of Glugea americanus to Spraguea americana comb. n. Since no major differences in ultrastructure or SSU rDNA sequence data exist between Spraguea americana and the microsporidian from the Japanese anglerfish, they evidently belong to the same species. This report of Spraguea americana is the first report of a Spraguea species from L. litulon and indeed from the Pacific water mass. Microsporidian parasites found in the nervous tissues of anglerfish of the genus Lophius were first reported by Thélohan (1895), who described the infection of the spinal ganglia of Lophius piscatorius (Linnaeus, 1758) collected from the Atlantic coast of France with a species of Glugea, but was unable to complete his description. Doflein (1898) found the same condition in L. piscatorius sampled from Italy, and as the parasite assemblages resembled those described by Moniez (1887) for Glugea anomala, infecting the musculature of the stickleback Gasterosteus aculeatus, the microsporidian from L. piscatorius was assigned to this genus an

Year: 2013
OAI identifier: oai:CiteSeerX.psu:10.1.1.330.8870
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