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An essential role for interleukin 10 in the function of regulatory T cells that inhibit intestinal inflammation

By Chrystelle Asseman, Smita Mauze, Michael W. Leach, Robert L. Coffman and Fiona Powrie

Abstract

A T helper cell type 1–mediated colitis develops in severe combined immunodeficient mice after transfer of CD45RB high CD4 � T cells and can be prevented by cotransfer of the CD45RB low subset. The immune-suppressive activities of the CD45RB low T cell population can be reversed in vivo by administration of an anti-transforming growth factor � antibody. Here we show that interleukin (IL)-10 is an essential mediator of the regulatory functions of the CD45RB low population. This population isolated from IL-10–deficient (IL-10 �/ � ) mice was unable to protect from colitis and when transferred alone to immune-deficient recipients induced colitis. Treatment with an anti–murine IL-10 receptor monoclonal antibody abrogated inhibition of colitis mediated by wild-type (WT) CD45RB low CD4 � cells, suggesting that IL-10 was necessary for the effector function of the regulatory T cell population. Inhibition of colitis by WT regulatory T cells was not dependent on IL-10 production by progeny of the CD45RB high CD4 � cells, as CD45RB low CD4 � cells from WT mice were able to inhibit colitis induced by IL-10 �/ � CD45RB high CD4 � cells. These findings provide the first clear evidence that IL-10 plays a nonredundant role in the functioning of regulatory T cells that control inflammatory responses towards intestinal antigens

Year: 1999
OAI identifier: oai:CiteSeerX.psu:10.1.1.328.2803
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