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By  and Maria L. UrsoAngus G. Scrimgeour, Yi-wen Chen, Paul D. Thompson, Priscilla M and Maria L. Urso

Abstract

We examined the effects of 48h of knee immobilization on alterations in mRNA and protein in human skeletal muscle. We hypothesized that 48h of immobilization would increase gene expression and respective protein products for ubiquitin proteasome pathway (UPP) components. Also, we used microarray analysis to identify novel pathways. Biopsies were taken from the vastus muscle of five men (20.4 + 0.5 years) before and after 48h immobilization. Global changes in gene expression were analyzed using Affymetrix GeneChips. Candidate genes were confirmed via quantitative real time polymerase chain reaction (qRT-PCR). Western blotting (WB) was used to quantify protein products of candidate genes and to assess Akt pathway activation. Immunohistochemistry (IHC) was used to localize proteins found to be altered when assessed via WB. The greatest percentage of genes showing altered expression with the GeneChip included genes involved in the UPP, metallothionein function, and extracellular matrix (ECM) integrity. qRT-PCR analysis confirmed increases in mRNA for UPP components (USP-6, SUMO-1) and the metallothioneins (MT2A, MT1F, MT1H, MT1X); and decreases in mRNA content for matrix metalloproteinases (MMP

Year: 2013
OAI identifier: oai:CiteSeerX.psu:10.1.1.325.2787
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