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Clinical Chemistry 53:7 1280–1288 (2007) Cancer Diagnostics Concentrations of TIMP1 mRNA Splice Variants and TIMP-1 Protein Are Differentially Associated with Prognosis in Primary Breast Cancer

By Anieta M. Sieuwerts, Pernille A. Usher, Marion E. Meijer-van Gelder, Mieke Timmermans, John W. M. Martens, Nils Brünner, Jan G. M. Klijn, Hanne Offenberg and John A. Foekens


Background: TIMP-1 protein is a prognostic factor for recurrence-free and overall survival (OS) time in breast cancer. We evaluated the prognostic value of TIMP1 mRNA and a novel TIMP1 mRNA splice variant in 1301 primary breast cancer patients. Methods: We measured mRNA transcripts of fulllength TIMP1 (TIMP1-v1) and the novel splice variant lacking exon 2 (TIMP1-v2) by use of real-time RT-PCR in frozen primary tumor samples. Transcript concentrations are correlated with histomorphological and biological factors, TIMP-1 protein, and distant metastasisfree survival (MFS) and OS time. Results: TIMP1-v1 and TIMP1-v2 alone were not informative with respect to predicting prognosis. However, the PCR assay designed to measure the combination o

Year: 2013
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