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Na,K-ATPase gene transfer mitigates an oxidant-induced decrease of active sodium transport in rat fetal ATII cells. Am J Respir Cell Mol Biol 2001;24:245–252

By Ulrich Thome, Lan Chen, Phillip Factor, Vidas Dumasius, Bruce Freeman, J. Iasha Sznajder and Sadis Matalon

Abstract

Abbreviations: recombinant replication-incompetent human type 5 adenovirus containing a cDNA for the �1 subunit of the rat Na,K-ATPase, ad�1; recombinant replication-incompetent human type 5 adenovirus containing a cDNA for the �1 subunit of the rat Na,K-ATPase, ad�1; analysis of variance, ANOVA; adult alveolar type 2 cells, ATII; cytopathologic effect by viral transfection, CPE; epithelial Na � channel, ENaC; fetal bovine serum, FBS; fetal distal lung epithelia, FDLE; homogenization buffer, HB; short circuit current, ISC; minimal essential medium, MEM; messenger RNA, mRNA; ouabain-sensitive component of the amphotericin-induced ISC, ouabmax; phosphate-buffered saline, PBS; polymerase chain reaction, PCR; plaque-forming units, pfu; standard deviation, SD; standard error of the mean, SEM. Am. J. Respir. Cell Mol. Biol. Vol. 24, pp. 245–252, 2001 Internet address: www.atsjournals.org We investigated whether adenovirus-mediated transfer of genes encoding for subunits of the Na,K-ATPase increases transepithelia

Year: 2013
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