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1,N 2 - propanodeoxyguanosine adducts: potential new biomarkers of smokinginduced DNA damage in human oral tissue

By Raghu G. Nath, Joseph E. Oc, Fung-lung Chung, Cancer Res, Contact The Aacr Publications, Raghu G. Nath, Joseph E. Oc and Fung-lung Chune

Abstract

Our previous study (R. G. Nath and F-L. Chung; Proc. Nail. Acad. Sci. USA, 91: 7491—7495, 1994), using a 32P postlabeling method combined with high-performance liquid chromatography specifically developed for exocyclic adducts, has shown that acrolein- and crotonaldehyde-derived 1,N2.propanodeoxyguanosine adducts (AdG and CdG, respectively) are present in the liver DNA from humans and rodents without carcinogen treatment. Those findings raised important questions regarding their role as potential endogenous DNA lesions in carcinogenesis. In this study, using a similar assay, we examined a variety of tissues from untreated rats and mice (lung, kidney, brain, breast, prostate, colon, skin, and leukocytes) and detected AdG and CdG in the DNA of these tissues. More signifi cantly, we also obtained evidence for the presence of these adducts in the DNA of human leukocytes and mammary glands. The identities of these adducts were verified by comigration of 3',5'.bisphosphates of the 32Plabele

Year: 1998
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