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TETRAHYMENA STRIVES TO MAINTAIN THE FLUIDITY INTERRELATIONSHIPS OF ALL ITS MEMBRANES CONSTANT Electron Microscope Evidence

By Yasuo Kitajima and Guy A. Thompson

Abstract

When cells of Tetrahymena pyriformis, strain NT-1, were chilled from their growth temperature of 39.5 ~ to lower temperatures, the plasma membrane, outer alveolar, nuclear, outer mitochondrial, food vacuolar, and endoplasmic reticulum membranes each responded in a fashion quite characteristic of the membrane type. In most cases a distinctive rearrangement of intramembrane particles, as discerned by freeze-fracture electron microscopy, began abruptly at a definitive temperature. By comparing the freeze-fracture patterns of membranes in cells grown at 39.5, 27, and 15 ~ it was shown that the initial particle rearrangement in a given membrane always occurred at a fixed number of degrees below the growth temperature of the cell. Gradual chilling of a cell grown at constant temperature induced these membrane changes first in the outer alveolar membrane, then, in order of decreasing response to temperature, in the endoplasmic reticulum, outer mitochondrial membrane, nuclear envelope, and vacuolar membrane. The normally stable relationships between the physical properties o

Year: 1977
OAI identifier: oai:CiteSeerX.psu:10.1.1.321.6525
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