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The Journal of Immunology Characterization of the Anti-Tissue Transglutaminase Antibody Response in Nonobese Diabetic Mice 1

By Daniele Sblattero, Francesco Maurano, Mauro Rossi, Salvatore Auricchio, Fiorella Florian, Fabiana Ziberna, Alberto Tommasini, Tarcisio Not, Ro Ventura, Andrew Bradbury, Roberto Marzari and Riccardo Troncone


Type 1 diabetes mellitus is an autoimmune disorder characterized by destruction of insulin-producing pancreatic � cells by T lymphocytes. In nonobese diabetic (NOD) mice, a role has been hypothesized for dietary gluten proteins in the onset of diabetes, and because gluten dependence is the major feature of celiac disease, together with production of Abs to the autoantigen tissue transglutaminase (tTG), we looked for the presence of anti-tTG Abs in the serum of NOD mice and, to establish their origin, analyzed the Ab repertoire of NOD mice using phage display Ab libraries. We found significant levels of serum anti-tTG Abs and were able to isolate single-chain Ab fragments to mouse tTG mainly from the Ab libraries made from intestinal lymphocytes and to a lesser extent from splenocytes. Data from NOD mice on a gluten-free diet suggest that the anti-tTG response is not glutendependent. The intestinal Ab response to tTG is a feature of NOD mice, but the underlying mechanisms remain obscure. The Journal of Immunology, 2005, 174: 5830–5836. Nonobese diabetic (NOD) 3 mice develop spontaneous autoimmune disorders including insulin-dependent or type 1 diabetes mellitus (T1DM), Sjögren’s syndrome, systemic lupus erythematosus, and arthritis (1). T1DM, the most characterized NOD disease, is the result of selective destruction of insulin-producing pancreatic � cells by autoimmune T lymphocyte

Year: 2013
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