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�1 and �3 Integrins Cooperate to Induce Syndecan-4-Containing Cross-linked Actin Networks

By In Human Trabecular Meshwork Cells, Mark S. Filla, Anne Woods, Paul L. Kaufman and Donna M. Peters


PURPOSE. To characterize the molecular composition of crosslinked actin networks (CLANs) and the regulation of their formation by integrins in normal human trabecular meshwork (TM) cells. CLANs have been observed in steroid-treated and glaucomatous TM cells and have been suggested to contribute to decreased outflow facility by altering the contractility of the TM. METHODS. Immunofluorescence microscopy was used to identify molecular components of CLANs and quantitate CLAN formation in HTM cells plated on coverslips coated with various extracellular matrix (ECM) proteins (fibronectin, types I and IV collagen, and vitronectin), vascular cell adhesion molecule (VCAM)-1, or activating antibodies against �1, �3, or �2�1 integrins. These integrin antibodies were also used as soluble ligands

Year: 2013
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