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Effect of TGF-�2 and Anti–TGF-�2 Antibody in a New In Vivo Rodent Model of Posterior Capsule Opacification

By Noemi Lois, Julie Taylor, Alastair D. Mckinnon, Gillian C. Smith, Rob Van’t Hof and John V. Forrester

Abstract

PURPOSE. This study evaluated the effect of transforming growth factor (TGF)-�2 and anti–TGF-�2 antibody in a rodent model of posterior capsule opacification (PCO). METHODS. An extracapsular lens extraction (ECLE) was performed in 72 Sprague–Dawley rats. At the end of the procedure, 10 �L TGF-�2 (TGF-�2–treated group), fetal calf serum (FCS)/phosphate-buffered saline (PBS; FCS/PBS-treated control group), a human monoclonal TGF-�2 antibody (anti–TGF-�2– treated group), or a null control IgG4 antibody (null antibody– treated control group) was injected into the capsule. Animals were killed 3 and 14 days postoperatively. Eyes were evaluated clinically prior to euthanatization, then enucleated and processed for light microscopy and immunohistochemistry afterward. PCO was evaluated clinically and histopathologically. Student’s t-test and � 2 were used to assess differences between groups. RESULTS. There were no statistically significant clinical or histopathological differences in degree of PCO between the TGF-�2 – and FCS/PBS-treated groups at 3 and 14 days after ECLE. Nor were there differences between the anti–TGF-�2 – and the null antibody–treated groups, with the exception of the histopathology score for capsule wrinkling 3 days after ECLE (P � 0.02). �-Smooth-muscle actin staining was observed in the lens capsular bag only in areas where there was close contact with the iris. CONCLUSIONS. No sustained effect of TGF-�2 or anti–TGF-�2 antibody on PCO was found in rodents at the dose and timing administered in this study. Iris cells may play a role in the process of epithelial mesenchymal transition linked to PCO

Topics: Scotland, United Kingdom
Year: 2005
OAI identifier: oai:CiteSeerX.psu:10.1.1.321.3344
Provided by: CiteSeerX
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