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Vitreous Modulation of Gene Expression in Low-Passage Human Retinal Pigment Epithelial Cells

By Ramapriya Ganti, Richard C. Hunt, Sunil K. Parapuram and D. Margaret Hunt


epithelial (RPE) cells enter the vitreous and proliferate. They become fibroblast-like and participate in the formation of contractile membranes, which can lead to retinal detachment. Vitreous treatment of RPE cells in vitro results in similar morphologic changes. This study was conducted to examine vitreous-induced modulation of gene expression in RPE cells. METHODS. Low-passage human RPE cell lines derived from three donors were each treated for 6, 12, 24, or 48 hours with complete medium or complete medium containing 25 % vitreous. Changes in mRNA levels were examined by using microarrays. Real-time quantitative PCR (qPCR) was used to measure mRNA expression of a subset of genes in cells from three additional donors. Immunohistochemistry and immunoblot analysis were used to examine protein expression. RESULTS. Vitreous treatment caused a progressive reprogrammin

Year: 2013
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