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Printed in U.S.A. Copyright © 2002 by The Endocrine Society Specific Detection of Type III Iodothyronine Deiodinase Protein in Chicken Cerebellar Purkinje Cells

By C. H. J. Verhoelst, K. Vandenborne, T. Severi, O. Bakker, B. Zandieh Doulabi, J. L. Leonard, E. R. Kühn, S. Van, Der Geyten and V. M. Darras

Abstract

Because iodothyronine deiodinases play a crucial role in the regulation of the available intracellular T 3 concentration, it is important to determine their cellular localization. In brain, the presence of type III iodothyronine deiodinase (D3) seems to be important to maintain homeostasis of T 3 levels. Until now, no cellular localization pattern of the D3 protein was reported in chicken brain. In this study polyclonal antisera were produced against specific peptides corresponding to the D3 amino acid sequence. Their use in immunocytochemistry led to the localization of D3 in the Purkinje cells of the chicken cerebellum. Both preimmune serum as well as the primary antiserum exhausted with the peptide itself were used as negative controls. Extracts of chick cerebellum and liver were made in the presence of Triton X-100 to solubilize the membrane-bound deiodinases. Using these extracts in Wester

Year: 2013
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