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Running head: Forskolin-induced LTP Corresponding author:

By Nikolai Otmakhov, Lena Khibnik, Nonna Otmakhova, Stephen Carpenter, Riahi Brent Asrican and Dr. John Lisman

Abstract

Copyright (c) 2003 by the American Physiological Society. 1 Chemically induced long-term potentiation (cLTP) could potentially work by directly stimulating the biochemical machinery that underlies synaptic plasticity, bypassing the need for synaptic activation. Previous reports suggested that agents that raise cAMP concentration might have this capability. We examined the cLTP induced in acute slices by application of Sp-cAMPS or a combination of the adenylyl cyclase activator, Forskolin, and the phosphodiesterase inhibitor, Rolipram. Under our conditions, cLTP was induced, but only if inhibition was reduced. We found that this form of cLTP was blocked by an NMDAR antagonist and required the low-frequency test stimulation typically used to monitor the strength of synapses. Interestingly, similar LTP could be induced by lowering the Mg 2+ concentration of the ACSF during Forskolin/Rolipram or Sp-cAMPS application or even by just lowering Mg 2+ concentration alone. This LTP was also NMDAR-dependent and required only a few (around five) low frequency stimuli for its induction. The finding that even low frequenc

Year: 2013
OAI identifier: oai:CiteSeerX.psu:10.1.1.319.7021
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