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Neurobiology of Disease Neuroimaging and Physiological Evidence for Involvement of Glutamatergic Transmission in Regulation of the Striatal

By Dopaminergic System, Masaki Tokunaga, Nicholas Seneca, Ryong-moon Shin, Jun Maeda, Shigeru Obayashi, Takashi Okauchi, Yuji Nagai, Ming-rong Zhang, Ryuji Nakao, Hiroshi Ito, Robert B. Innis, Christer Halldin, Kazutoshi Suzuki, Makoto Higuchi and Tetsuya Suhara


Aberrant neurotransmissions via glutamate and dopamine receptors have been the focus of biomedical research on the molecular basis of psychiatric disorders, but the mode of their interaction is yet to be uncovered. In this study, we demonstrated the pharmacological reversal of methamphetamine-stimulated dopaminergic overflow by suppression of group I metabotropic glutamate (mGlu) receptor in living primates and rodents. In vivo positron emission tomography (PET) was conducted on cynomolgus monkeys and rats using a full agonistic tracer for dopamine D2/3 receptor, [ 11 C]MNPA [(R)-2- 11 CH3O-N-n-propylnorapomorphine], and fluctuation of kinetic data resulting from anesthesia was avoided by scanning awake subjects. Excessive release of dopamine induced by methamphetamine and abolishment of this alteration by treatment with an antagonist of group I mGlu receptors, 2-methyl-6-(phenylethynyl)pyridine (MPEP), were measured in both species as decreased binding potential because of increased dopamine and its recovery to baseline levels, respectively. Counteraction of MPEP to the methamphetamine-induced dopamine spillover was also supported neurochemically by microdialysis of unanesthetized rat striatum. Moreover, patch-clamp electrophysiological assays using acute brain slices prepared from rats indicated that direct targets of MPEP mechanistically involved in the effects of methamphetamine are present locally within the striatum. Because MPEP alone did not markedly alter the baseline dopaminergic neurotransmission according to our PET and electrophysiologica

Year: 1887
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