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Evaluation of Current Peptide Production Platforms

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Abstract

ics experiments, pattern reproducibility within and across experiments in a single group and between groups becomes a major challenge. Absolute quantification, on the other hand, does not have any of the above limitations (10) because it allows comparison of data from any experiment (no reference/ control needed), provides boundary conditions for different experiments, and makes data comparable between laboratories. In addition, absolute quantification is especially important in the biomarker discovery work flow because it provides necessary information for assay development. The gold standard for absolute quantification of proteins and peptides is amino acid analysis. Unfortunately this method is slow and requires considerable amounts of starting material that is destroyed in the analysis and knowledge of the protein or peptide sequence (11). In addition, the quantifie

Year: 2013
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