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Mapping Late Blight Resistance in Solanum microdontum Bitter

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Abstract

foliar late blight resistance in other populations (Collins A diploid Solanum population was developed with the objective et al., 1999; Sandbrink et al., 2000). Association between of mapping quantitative trait loci (QTL) associated with resistance QTL conferring foliar late blight resistance, tuberiza-to Phytophthora infestans (Mont.) de Bary, the causal organism of tion, and vine maturity was found in four out of five late blight. The mapping population was a cross between a late blight chromosomes (Ewing et al., 2000). The knowledge about resistant selection of Solanum microdontum Bitter and a susceptible late blight resistance associations with undesirable traits diploid breeding clone. The progeny of 109 clones and the parents is also of value before introgressing a new source of rewere field tested for foliar late blight reaction in 1999 and 2000 and sistance. for vine maturity in 2000 and 2001. Parents and progeny were geno- The South American diploid species S. microdontum typed with isozymes and simple sequence repeats (SSR) markers. A has shown high levels of resistance to late blight (Colon total of 161 pairs of SSR primers were screened, from which 74 ampli- and Budding, 1988; Colon et al., 1995a, 1995c; Douches fied polymorphic bands in Metaphor agarose or polyacrylamide gels et al., 2001). Among 618 clones representing 24 accesyielding a total of 109 SSR loci. The isozyme and SSR combine

Year: 2013
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