Abstract. In yeast, actin forms patches associated with the plasma membrane. Patch distribution correlates with polarized growth during the cell cycle and in response to external stimuli. Using green fluorescent protein fused to capping protein to image actin patches in living cells, we find that patches move rapidly and over long distances. Even patches in clusters, such as at the incipient bud site, show movement. Patches move inde-I r ~ yeast, actin functions in polarized secretion and growth (6, 37). Actin filaments in yeast are found in cortical patches associated with invaginations of the plasma membrane and in cables running through the cytoplasm (26). The polarization of patches and the orientation of cables correlates with polarized cell growth (1). The distribution of both patches and cables changes dramaticall
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