We have studied the expression of insulin-like growth fador type II (IGF-ll) and its autocrine role during the proliferation and differentiation of the CaCo-2 colon carcinoma cell line. ICE-Il RNA levels were high in proliferating cells and decreased by more than 1 0-fold when cells ceased to proliferate and differentiated. Immunoreadive ICE-Il protein was high in the conditioned media of proliferating cells and decreased 20-fold in the media of differentiated cells. Reduced ICE-Il expression was associated with a decrease in ICE-I receptor number that was high in proliferating cells (approximately 80,000 binding sites/cell) and reduced by 4-fold in differentiated cells. Exogenously added ICE-Il was able to stimulate proliferation o
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